Liquid-liquid phase separation: Galectin-3 in nuclear speckles and ribonucleoprotein complexes.
Liquid-liquid phase separation: Galectin-3 in nuclear speckles and ribonucleoprotein complexes.
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DOI：
10.1016/j.yexcr.2023.113571
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发表时间：
2023-06-01
影响因子：
3.7
通讯作者：
Wang, John L.
中科院分区：
文献类型：
--
作者：
Voss, Patricia G.;Wang, John L.
关键词：
核斑点是亚细胞结构，最初通过单克隆抗体SC 35的点状免疫荧光染色表征，其识别SRRM 2（丝氨酸/精氨酸重复基质蛋白2）和Sfrs 2（剪接因子SR（富含丝氨酸/精氨酸）家族的成员）上的表位。半乳糖凝集素-3与SC 35共定位于核斑点中，其代表一组核体，包括核仁、Cajal体和宝石、副斑点等。虽然它们似乎具有良好的物理边界，但这些核体不是膜结合结构，而是代表由称为液-液相分离的现象产生的大分子组装体。近年来，液相凝聚作为一种新认识到的细胞组织和划分具有不同组成的亚细胞结构的机制引起了人们的极大兴趣。半乳糖凝集素-3与SC 3的点状/斑点染色证明了它们在细胞内源性条件下在体内相分离体内的共定位。本综述的目的是总结的研究，文件的三个关键特征的半乳糖凝集素-3在液相冷凝物中的本地化：（a）一个内在的无序结构域;（B）寡聚体形成多价结合;（c）与RNA和核糖核蛋白复合物的协会。
Nuclear speckles are subcellular structures originally characterized by punctate immunofluorescence staining of the monoclonal antibody SC35, which recognizes an epitope on SRRM2 (serine/arginine repetitive matrix protein 2) and Sfrs2, a member of the SR (serine/arginine-rich) family of splicing factors. Galectin-3 co-localizes with SC35 in nuclear speckles, which represent one group of nuclear bodies that include the nucleolus, Cajal bodies and gems, paraspeckles, etc. Although they appear to have well-delineated physical boundaries, these nuclear bodies are not membrane-bound structures but represent macromolecular assemblies arising from a phenomenon called liquid-liquid phase separation. There has been much recent interest in liquid phase condensation as a newly recognized mechanism by which a cell can organize and compartmentalize subcellular structures with distinct composition. The punctate/speckled staining of galectin-3 with SC3 demonstrates their co-localization in a phase-separated body in vivo, under conditions endogenous to the cell. The purpose of the present review is to summarize the studies that document three key features of galectin-3 for its localization in liquid phase condensates: (a) an intrinsically disordered domain; (b) oligomer formation for multivalent binding; and (c) association with RNA and ribonucleoprotein complexes.
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DOI：
10.1083/jcb.200604009
发表时间：
2006-10-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Dellaire G;Ching RW;Ahmed K;Jalali F;Tse KC;Bristow RG;Bazett-Jones DP
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影响因子：
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作者：
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DOI：
10.1083/jcb.201303145
发表时间：
2013-10-14
期刊：
The Journal of cell biology
影响因子：
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作者：
Fong KW;Li Y;Wang W;Ma W;Li K;Qi RZ;Liu D;Songyang Z;Chen J
通讯作者：
Chen J
影响因子：
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作者：
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通讯作者：
SCHWARTZ, LB
影响因子：
56.9
作者：
Brangwynne, Clifford P.;Eckmann, Christian R.;Hyman, Anthony A.
通讯作者：
Hyman, Anthony A.