Millisecond Imaging of Neural Responses to Micro-stimuli in the Visual Cortex Layer II/III In Vitro
Millisecond Imaging of Neural Responses to Micro-stimuli in the Visual Cortex Layer II/III In Vitro
复制标题
体外视觉皮层 II/III 层微刺激神经反应的毫秒成像
DOI：
10.1109/lifetech48969.2020.1570619123
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Y. Hayashida
中科院分区：
文献类型：
--
作者：
S. Hayashi;K. Yoshida;T. Nomoto;Y. Tanaka;T. Yagi;Y. Hayashida
揭示皮层对微刺激的时空反应的定量性质对皮质内神经假体的设计具有重要意义。尽管皮层中的神经元尖峰和突触信号具有毫秒级的精度，但在这种时间分辨率下的电路响应动力学研究仍然有限。目前，采用电压敏感染料成像技术，在小鼠大脑切片上观察了皮层神经对重复微刺激脉冲的反应。当刺激脉冲间间隔为5 msec时，直接由刺激脉冲引起的群体峰幅值依次下降，而非20 msec。这与实验之间的相似之处在于，刺激应用于第II/III层或第IV层。这些结果表明，在群体水平上的峰值不应期是设计重复脉冲刺激有效频率的限制条件之一。
Unraveling the quantitative properties of the spatio-temporal cortical responses to microstimulation is of essential importance for designing intracortical neural prostheses. Despite the millisecond-order precision of neuronal spiking and synaptic signaling in the cortex, the circuit response dynamics at such temporal resolution has been investigated to a limited extent. In the present, cortical neural responses to repetitive microstimulation pulses were examined in the mouse cerebral slices by means of the voltage-sensitive dye imaging. The amplitude of the population spikes induced directly by the stimulus pulses successively decreased when the inter-pulse interval of the stimuli was 5 msec, but not 20 msec. This was similar between the experiments in that the stimulation was applied to either the layer II/III or IV. These results suggested that the refractory period of spiking at the population level is one of limiting conditions for designing efficient frequency for repetitive pulse stimulation.
影响因子：
4
作者：
Yuta Tanaka;Tomohiro Nomoto;Takuya Shiki;Y. Sakata;Yoshinori Shimada;Y. Hayashida;T. Yagi
通讯作者：
Yuta Tanaka;Tomohiro Nomoto;Takuya Shiki;Y. Sakata;Yoshinori Shimada;Y. Hayashida;T. Yagi
影响因子：
3.3
作者：
E. J. Tehovnik;W. Slocum
通讯作者：
E. J. Tehovnik;W. Slocum