Selective patterning of Si-based biosensor surfaces using isotropic silicon etchants.
Selective patterning of Si-based biosensor surfaces using isotropic silicon etchants.
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DOI：
10.1016/j.jcis.2011.11.082
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发表时间：
2012-03-01
影响因子：
9.9
通讯作者：
Armani, Andrea M.
中科院分区：
文献类型：
--
作者：
Biggs, Bradley W.;Hunt, Heather K.;Armani, Andrea M.
超灵敏、无标记的生物传感器有可能对医疗诊断等领域产生巨大影响。对于大多数这些Si基集成器件，有必要用靶向配体使表面功能化，以便进行两种特异性生物检测。为此，硅烷偶联剂通常用于修饰靶向配体。然而，该方法通常导致整个装置表面的生物缀合，这是不期望的。为了补偿这种影响，研究人员开发了复杂的阻断策略，导致传感器表面的选择性图案化。最近，硅烷偶联剂被用于将生物分子附着到集成在硅晶片上的二氧化硅环形生物传感器的表面。有趣的是，只有二氧化硅生物传感器表面是共轭的。在这里，我们假设为什么会发生这种选择性模式。具体而言，用于制造生物传感器的硅蚀刻剂（二氟化氙）似乎降低了硅烷偶联附着到下面的硅晶片的效率。这些结果将使未来的研究人员能够更容易地控制其传感器表面的生物共轭，从而提高生物传感器设备的性能。
Ultra-sensitive, label-free biosensors have the potential to have a tremendous impact on fields like medical diagnostics. For the majority of these Si-based integrated devices, it is necessary to functionalize the surface with a targeting ligand in order to perform both specific biodetection. To do this, silane coupling agents are commonly used to immobilize the targeting ligand. However, this method typically results in the bioconjugation of the entire device surface, which is undesirable. To compensate for this effect, researchers have developed complex blocking strategies that result in selective patterning of the sensor surface. Recently, silane coupling agents were used to attach biomolecules to the surface of silica toroidal biosensors integrated on a silicon wafer. Interestingly, only the silica biosensor surface was conjugated. Here, we hypothesize why this selective patterning occurred. Specifically, the silicon etchant (xenon difluoride), which is used in the fabrication of the biosensor, appears to reduce the efficiency of the silane coupling attachment to the underlying silicon wafer. These results will enable future researchers to more easily control the bioconjugation of their sensor surfaces, thus improving biosensor device performance.
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