The effects of prolactin and gonadotropin on luteal function and morphology in the cyclic golden hamster.
The effects of prolactin and gonadotropin on luteal function and morphology in the cyclic golden hamster.
复制标题
催乳素和促性腺激素对周期性金仓鼠黄体功能和形态的影响。
DOI：
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发表时间：
2008
影响因子：
1.8
通讯作者：
K. Taya
中科院分区：
文献类型：
--
作者：
H. Kon;H. Kishi;K. Arai;M. Shinoda;G. Watanabe;K. Taya
本研究旨在评价垂体对周期性金黄地鼠（Mesocritus auratus）黄体维持和退化的内分泌作用。在发情周期第1天（排卵日）0900 h切除垂体（Hypox）后，动物接受催乳素（PRL）或PRL+马绒毛膜促性腺激素（eCG）注射。在周期第3天1500 h断头取躯干血测定孕酮（P4）。从一侧卵巢中分离黄体，电泳检测DNA梯状条带，荧光法测定DNA片段化率，并测定P4。另一侧卵巢用于组织学观察。缺氧后，每天注射1mg催乳素可抑制CLs的DNA断裂率和凋亡细胞数。PRL处理维持了黄体形态，并增加了黄体P4浓度，但不增加血浆P4浓度。除PRL外，在Hypox后注射2 IU eCG也将CL中的DNA断裂率和凋亡细胞数量抑制到妊娠动物的水平。PRL加eCG处理以与仅PRL处理相同的方式维持黄体形态，并且不仅增加黄体而且增加血浆P4浓度。这些结果表明，PRL抑制黄体细胞凋亡，维持黄体形态，PRL和eCG的组合抑制不仅结构，而且功能性黄体退化的周期仓鼠。
The present study was conducted to evaluate the endocrinological effects of the pituitary on luteal maintenance and regression in the cyclic golden hamster (Mesocritus auratus). After hypophysectomy (Hypox) at 0900 h on day 1 of the estrous cycle (the day of ovulation), the animals received injection of prolactin (PRL) or PRL plus equine chorionic gonadotropin (eCG). They were decapitated at 1500 h on day 3 of the cycle, and trunk blood was collected for measurement of progesterone (P4). Corpora lutea (CLs) were dissected from one ovary for DNA ladder detection by electrophoresis, determination of DNA fragmentation ratio by fluorometric measurement method and measurement of P4. The other ovary was used for histological observation. After the Hypox, the daily injection of 1 mg ovine PRL restrained the DNA fragmentation ratio and number of apoptotic cell in the CLs. The PRL treatment maintained the luteal morphology and increased the luteal P4 concentration, but not in the plasma P4 concentration. In addition to PRL, injection of 2 IU eCG after the Hypox also restrained the DNA fragmentation ratio and number of apoptotic cells in the CLs to the level of a pregnant animal. The PRL plus eCG treatment maintained the luteal morphology in the same manner as the PRL only treatment and increased not only the luteal but also the plasma P4 concentration. These results suggest that PRL restrains luteal apoptosis and maintains luteal morphology and that the combination of PRL and eCG restrains not only structural but also functional luteal regression in the cyclic hamster.
影响因子：
3.6
作者：
J. T. McCormack;M. Friederichs;S. D. Limback;G. S. Greenwald
通讯作者：
J. T. McCormack;M. Friederichs;S. D. Limback;G. S. Greenwald
伽马射线照射的基因诱导导致淋巴细胞中的 DNA 断裂。
DOI：
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发表时间：
1987
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Sellins,KS;Cohen,JJ
通讯作者：
Cohen,JJ
影响因子：
3.6
作者：
Bo R. Rueda;Julie A. Wegner;S. Marion;Derron D. Wahlen;Patricia B. Hoyer
通讯作者：
Bo R. Rueda;Julie A. Wegner;S. Marion;Derron D. Wahlen;Patricia B. Hoyer