Evolution of the sex-determining region in Ginkgo biloba.
Evolution of the sex-determining region in Ginkgo biloba.
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DOI：
10.1098/rstb.2021.0229
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发表时间：
2022-05-09
期刊：
影响因子：
--
通讯作者：
Filatov DA
中科院分区：
文献类型：
--
作者：
Gong W;Filatov DA
性染色体或性别决定区（SDR）已在许多雌雄异株植物物种中被发现，包括标志性的“活化石”银杏，尽管SDR在G. biloba中的位置和大小仍然存在矛盾。在此，我们解决了这些争议，并分析了SDR在该物种中的演变。基于四个遗传杂交的转录组测序数据，我们重建了雄性和雌性特异性遗传图谱，并将SDR定位在2号染色体中间。遗传图谱与基因组序列的整合显示，在男性和女性中，SDR及其周围的重组在约50 Mb的区域内受到抑制。然而，偶尔会发生重组，除了一个小的，小于5mb长的区域，在雄性中不重组。根据该区域同源X-和y -连锁基因的同义分化，我们推断银杏SDR相当古老，至少起源于白垩纪。替代率和基因表达分析显示只有轻微的y变性。这些结果与其他具有同态性染色体的雌雄异株植物的发现一致，其中SDR通常很小，并且在预先存在的重组减少的区域中进化，周围是长时间活跃重组的假常染色体区域。本文是陆生植物性别决定与性染色体进化专题的一部分。
Sex chromosomes or sex-determining regions (SDR) have been discovered in many dioecious plant species, including the iconic ‘living fossil' Ginkgo biloba, though the location and size of the SDR in G. biloba remain contradictory. Here we resolve these controversies and analyse the evolution of the SDR in this species. Based on transcriptome sequencing data from four genetic crosses we reconstruct male- and female-specific genetic maps and locate the SDR to the middle of chromosome 2. Integration of the genetic maps with the genome sequence reveals that recombination in and around the SDR is suppressed in a region of about 50 Mb in both males and females. However, occasional recombination does occur except a small, less than 5 Mb long region that does not recombine in males. Based on synonymous divergence between homologous X- and Y-linked genes in this region, we infer that the Ginkgo SDR is fairly old—at least of Cretaceous origin. The analysis of substitution rates and gene expression reveals only slight Y-degeneration. These results are consistent with findings in other dioecious plants with homomorphic sex chromosomes, where the SDR is typically small and evolves in a region with pre-existing reduced recombination, surrounded by long actively recombining pseudoautosomal regions. This article is part of the theme issue ‘Sex determination and sex chromosome evolution in land plants’.
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DOI：
10.1093/bioinformatics/btp572
发表时间：
2009-12-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Filatov DA
通讯作者：
Filatov DA
影响因子：
64.8
作者：
Hughes, Jennifer F.;Skaletsky, Helen;Brown, Laura G.;Pyntikova, Tatyana;Graves, Tina;Fulton, Robert S.;Dugan, Shannon;Ding, Yan;Buhay, Christian J.;Kremitzki, Colin;Wang, Qiaoyan;Shen, Hua;Holder, Michael;Villasana, Donna;Nazareth, Lynne V.;Cree, Andrew;Courtney, Laura;Veizer, Joelle;Kotkiewicz, Holland;Cho, Ting-Jan;Koutseva, Natalia;Rozen, Steve;Muzny, Donna M.;Warren, Wesley C.;Gibbs, Richard A.;Wilson, Richard K.;Page, David C.
通讯作者：
Page, David C.
DOI：
10.1098/rstb.2000.0717
发表时间：
2000-11-29
影响因子：
6.3
作者：
Charlesworth, B;Charlesworth, D
通讯作者：
Charlesworth, D
影响因子：
9.2
作者：
Krasovec, Marc;Chester, Michael;Filatov, Dmitry A.
通讯作者：
Filatov, Dmitry A.
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro