Exogenous Selenium Treatment Promotes Glucosinolate and Glucoraphanin Accumulation in Broccoli by Activating Their Biosynthesis and Transport Pathways
Exogenous Selenium Treatment Promotes Glucosinolate and Glucoraphanin Accumulation in Broccoli by Activating Their Biosynthesis and Transport Pathways
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外源硒处理通过激活西兰花的生物合成和运输途径促进芥子油苷和萝卜硫苷的积累
DOI：
10.3390/app12094101
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发表时间：
2022-04
期刊：
影响因子：
--
通讯作者：
Ke Huang
中科院分区：
文献类型：
--
作者：
Qi Wu;Junwei Wang;Huiping Huang;Shuxiang Mao;Qiuyun Wu;Ke Huang
植物补硒是一种有效且广泛应用的方法。它不仅可以转化为更多的富硒化合物，还可以促进具有抗癌特性的硫代葡萄糖苷（GSL）的积累。然而，硒调节GSL合成的分子机制尚不清楚。在本研究中，我们分析了硒处理（50 μM亚硒酸钠）对青花菜组织中GSL、萝卜硫素（4M3）和萝卜硫素化合物的影响。还评估了参与硫吸收和运输、GSL生物合成、易位和降解途径的基因的转录水平。结果表明，硒处理显著促进了根中总硫和总硒含量的积累，使根中色氨酸源GSL含量提高了2倍。硒处理后，鲜叶中的4M3浓度和萝卜硫素含量分别增加了67%和30%。在基因表达方面，硒对硫酸盐吸收和转运蛋白、GSL生物合成和转运蛋白等相关基因的表达有明显的诱导作用。结果表明，外源硒处理通过激活硫吸收、GSLs合成和转运途径相关基因的转录水平，促进青花菜GSLs和4M硫含量的过量积累。
Supplementation using selenium (Se) on plants is an effective and widely used approach. It can not only be converted to more Se rich compounds but promote the accumulation of glucosinolates (GSLs) with anti-carcinogenic properties. However, the molecular mechanism of Se in regulating GSLs synthesis remains unclear. In the present study, we analyzed the effects of Se treatment (50 μM sodium selenite) on GSLs, glucoraphanin (4MSOB), and sulforaphane compounds in broccoli tissues. The transcript levels of genes involved in sulfur absorption and transport, GSLs biosynthesis, translocation, and degradation pathways were also evaluated. The study showed that Se treatment remarkably promoted the accumulation of total sulfur and total Se contents and increased Trp-derived GSLs levels in roots by 2 times. The 4MSOB concentration and sulforaphane content in fresh leaves was increased by 67% and 30% after Se treatment, respectively. For genes expressions, some genes involved in sulfate uptake and transporters, GSLs biosynthesis, and transporters were induced strongly upon Se exposure. Results revealed that exogenous Se treatment promotes the overaccumulation of GSLs and 4MSOB content in broccoli by activating the transcript levels of genes involved in sulfur absorption, GSLs biosynthesis, and translocation pathways.
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影响因子：
6.1
作者：
D'Amato R;Regni L;Falcinelli B;Mattioli S;Benincasa P;Dal Bosco A;Pacheco P;Proietti P;Troni E;Santi C;Businelli D
通讯作者：
Businelli D
影响因子：
2.1
作者：
Huang, K.;Lin, J. C.;Xiao, W. J.
通讯作者：
Xiao, W. J.
影响因子：
13.8
作者：
Yang J;Wang J;Li Z;Li X;He Z;Zhang L;Sha T;Lyu X;Chen S;Gu Y;Li Z;Hu Z;He H;Bancroft I;Zhang M
通讯作者：
Zhang M
影响因子：
7.4
作者：
Madsen, Svend Roesen;Olsen, Carl Erik;Halkier, Barbara Ann
通讯作者：
Halkier, Barbara Ann
影响因子：
11.6
作者：
Andersen, Tonni Grube;Nour-Eldin, Hussam Hassan;Halkier, Barbara Ann
通讯作者：
Halkier, Barbara Ann