A proteomic study on a human osteosarcoma cell line Saos-2 treated with diallyl trisulfide
A proteomic study on a human osteosarcoma cell line Saos-2 treated with diallyl trisulfide
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二烯丙基三硫化物处理人骨肉瘤细胞系 Saos-2 的蛋白质组学研究
DOI：
10.1097/cad.0b013e32832e89c7
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发表时间：
2009-09
期刊：
影响因子：
--
通讯作者：
Dong Mei Diao
中科院分区：
文献类型：
--
作者：
Yong Kui Zhang;Xu Hua Zhang;Jian Min Li;De Sheng Sun;Qiang Yang;Dong Mei Diao
大蒜通常被用作治疗各种疾病的治疗剂，许多研究表明，大蒜及其衍生物可以降低各种人类癌症的风险。二烯丙基三硫（DATS）是大蒜衍生物的主要成分，大量研究表明，DATS可通过诱导多种肿瘤细胞周期阻滞或凋亡抑制细胞增殖。然而，DATS是否对人骨肉瘤细胞具有相同的作用仍不清楚。在这项研究中，我们试图分析DATS对人骨肉瘤细胞系Saos-2细胞的细胞增殖，细胞周期，诱导凋亡，全球蛋白表达模式的影响，以及DATS作用的潜在分子机制。用25、50和100 μmol/l DATS处理人骨肉瘤细胞系Saos-2细胞不同时间间隔。本研究检测了细胞增殖、细胞周期进程和凋亡。然后，用50 μmol/lDATS处理Saos-2细胞48 h后，用双向电泳和质谱技术系统地研究了细胞内蛋白质的添加模式。DATS能抑制Saos-2细胞的增殖，并呈剂量和时间依赖性。此外，DATS处理后，凋亡细胞百分比和G 0/G1期细胞阻滞也呈剂量依赖性和时间依赖性。在Saos-2细胞中共检测到27种独特的蛋白质，包括18种下调蛋白质和9种上调蛋白质，其表达水平对应于DATS给药的显著变化。有趣的是，这些蛋白质中几乎有一半（27个中的13个）与细胞周期或凋亡有关。DATS通过阻断细胞周期进程和诱导细胞凋亡抑制Saos-2细胞的增殖，并呈剂量和时间依赖性。因此，蛋白质组学的结果，提供了额外的支持的假设，DATS是一个强大的诱导肿瘤细胞凋亡。然而，确切的分子机制，这些蛋白质如何显着改变Saos-2细胞系后，DATS处理，应进一步研究。
Garlic is generally used as a therapeutic reagent against various diseases, and numerous studies have indicated that garlic and its derivatives can reduce the risk of various types of human cancer. Diallyl trisulfide (DATS), a major member of garlic derivatives, could inhibit the cell proliferation by triggering either cell cycle arrest or apoptosis in a variety of cancer cell lines as shown in many studies. However, whether DATS has the same effect on human osteosarcoma cells remains unknown. In this study, we have attempted to analyze the effects of DATS on cell proliferation, cell cycle, induction of apoptosis, global protein expression pattern in a human osteosarcoma cell line Saos-2 cells, and the potential molecular mechanisms of the action of DATS. Saos-2 cells, a human osteosarcoma cell line, were treated with or without 25, 50, and 100 μmol/l DATS for various time intervals. The cell proliferation, cell cycle progression, and apoptosis were examined in this study. Then, after treatment with or without 50 μmol/l DATS for 48 h, protein add pattern in Saos-2 cells were systematically studied using two-dimensional electrophoresis and mass spectrometry. DATS could inhibit the proliferation of Saos-2 cells in a dose-dependent and time-dependent manner. Moreover, the percentage of apoptotic cell and cell arrest in G0/G1 phase was also dose-dependent and time-dependent upon DATS treatment. A total of 27 unique proteins in Saos-2 cells, including 18 downregulated proteins and nine upregulated proteins, were detected with significant changes in their expression levels corresponding to DATS administration. Interestingly, almost half of these proteins (13 of 27) are related to either the cell cycle or apoptosis. DATS has the ability to suppress cell proliferation of Saos-2 cells by blocking cell cycle progression and inducing apoptosis in a dose and time-dependent manner. The proteomic results presented, therefore, provide additional support to the hypothesis that DATS is a strong inducer of apoptosis in tumor cells. However, the exact molecular mechanisms, how these proteins significantly changed in the Saos-2 cell line upon DATS treatment, should be further studied.
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影响因子：
3.4
作者：
Spreafico, Adriano;Frediani, Bruno;Santucci, Annalisa
通讯作者：
Santucci, Annalisa
影响因子：
11.2
作者：
Itoh, Masahiko;Nelson, Celeste M.;Bissell, Mina J.
通讯作者：
Bissell, Mina J.
影响因子：
11.2
作者：
Sadmeet Singh;Skanda K. Sadacharan;S. Su;A. Belldegrun;S. Persad;G. Singh
通讯作者：
Sadmeet Singh;Skanda K. Sadacharan;S. Su;A. Belldegrun;S. Persad;G. Singh
影响因子：
4.4
作者：
Kang, Jeong Han;Park, Kwan-Kyu;Chang, Young-Chae
通讯作者：
Chang, Young-Chae
影响因子：
4.2
作者：
Rivlin, RS
通讯作者：
Rivlin, RS