Evolution of optimal growth temperature in Asgard archaea inferred from the temperature dependence of GDP binding to EF-1A.

Evolution of optimal growth temperature in Asgard archaea inferred from the temperature dependence of GDP binding to EF-1A.
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DOI:
10.1038/s41467-024-44806-1
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发表时间:
2024-01-15
影响因子:
16.6
通讯作者:
Li, Meng
Li, Meng
中科院分区:
综合性期刊1区
文献类型:
--
作者:
Lu, Zhongyi;Xia, Runyue;Zhang, Siyu;Pan, Jie;Liu, Yang;Wolf, Yuri I.;Koonin, Eugene V.;Li, Meng

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真核生物的远古祖先似乎属于阿斯加德门,但对阿加德古菌的生态和进化知之甚少。翻译延伸因子的最佳GDP结合温度(EF-1A或EF-Tu)已被证明与各种原核生物的最佳生长温度相关。在这里,我们重建了祖先的EF-1A序列,并通过实验测量了古代和现存的Asgard古菌EF-1A的最适GDP结合温度,以推断Asgardarchaeota的最适生长温度的进化。我们的结果表明,真核生物的祖先阿斯加德是一种中等嗜热性的生物,最适生长温度在53 °C左右。真核生物的起源似乎与阿斯加德古菌进化过程中从嗜热生活方式向中温生活方式的转变相吻合。真核生物的远古祖先属于阿斯加德古生物门,或阿斯加德古生菌。在这里,作者使用祖先序列重建和实验确定来自古代和现存的阿斯加德古菌的翻译延伸因子的最佳GDP结合温度,以推断真核生物祖先的最佳生长温度。
The archaeal ancestor of eukaryotes apparently belonged to the phylum Asgardarchaeota, but the ecology and evolution of Asgard archaea are poorly understood. The optimal GDP-binding temperature of a translation elongation factor (EF-1A or EF-Tu) has been previously shown to correlate with the optimal growth temperature of diverse prokaryotes. Here, we reconstruct ancestral EF-1A sequences and experimentally measure the optimal GDP-binding temperature of EF-1A from ancient and extant Asgard archaea, to infer the evolution of optimal growth temperatures in Asgardarchaeota. Our results suggest that the Asgard ancestor of eukaryotes was a moderate thermophile, with an optimal growth temperature around 53 °C. The origin of eukaryotes appears to coincide with a transition from thermophilic to mesophilic lifestyle during the evolution of Asgard archaea. The archaeal ancestor of eukaryotes belonged to the phylum Asgardarchaeota, or Asgard archaea. Here, the authors use ancestral sequence reconstruction and experimentally determine the optimal GDP-binding temperature of a translation elongation factor from ancient and extant Asgard archaea, to infer optimal growth temperatures for eukaryotes’ ancestors.
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