The wheat Sr22, Sr33, Sr35 and Sr45 genes confer resistance against stem rust in barley.
The wheat Sr22, Sr33, Sr35 and Sr45 genes confer resistance against stem rust in barley.
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小麦Sr22、Sr33、Sr35和Sr45基因赋予大麦对茎锈病的抗性。
DOI：
10.1111/pbi.13460
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发表时间：
2021-03
影响因子：
13.8
通讯作者：
Wulff BBH
中科院分区：
文献类型：
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作者：
Hatta MAM;Arora S;Ghosh S;Matny O;Smedley MA;Yu G;Chakraborty S;Bhatt D;Xia X;Steuernagel B;Richardson T;Mago R;Lagudah ES;Patron NJ;Ayliffe M;Rouse MN;Harwood WA;Periyannan S;Steffenson BJ;Wulff BBH
在过去的20年里，由禾柄锈菌（Puccinia graminis f.）在非洲和欧洲，小麦和大麦生产的主要威胁重新出现。与小麦的60个抗秆锈基因相比，大麦的抗秆锈遗传变异很小，仅鉴定出10个抗秆锈基因。其中，只有一个由三个基因组成的复杂基因座对TTKSK有效，TTKSK是1999年在乌干达出现的Ug99部落的一种广泛致命的Pgt种族，此后传播到东非大部分地区和中东部分地区。本研究的目的是评估功能，在大麦中，克隆的小麦Sr基因有效地对抗小种TTKSK。Sr22、Sr33、Sr35和Sr45分别转化大麦cv. Golden Promise使用农杆菌介导的转化。发现所有四个基因都赋予有效的茎锈病抗性。大麦转基因仍然对大麦叶锈病病原体大麦柄锈菌（Puccinia hordei）敏感，表明这些小麦Sr基因赋予的抗性是Pgt特异的。此外，这些转基因植物在没有疾病的情况下没有表现出显著的不利农艺效应。因此，从小麦中克隆的Sr基因是大麦抗小麦秆锈病的潜在来源。
In the last 20 years, stem rust caused by the fungus Puccinia graminis f. sp. tritici (Pgt), has re‐emerged as a major threat to wheat and barley production in Africa and Europe. In contrast to wheat with 60 designated stem rust (Sr) resistance genes, barley’s genetic variation for stem rust resistance is very narrow with only ten resistance genes genetically identified. Of these, only one complex locus consisting of three genes is effective against TTKSK, a widely virulent Pgt race of the Ug99 tribe which emerged in Uganda in 1999 and has since spread to much of East Africa and parts of the Middle East. The objective of this study was to assess the functionality, in barley, of cloned wheat Sr genes effective against race TTKSK. Sr22, Sr33, Sr35 and Sr45 were transformed into barley cv. Golden Promise using Agrobacterium‐mediated transformation. All four genes were found to confer effective stem rust resistance. The barley transgenics remained susceptible to the barley leaf rust pathogen Puccinia hordei, indicating that the resistance conferred by these wheat Sr genes was specific for Pgt. Furthermore, these transgenic plants did not display significant adverse agronomic effects in the absence of disease. Cloned Sr genes from wheat are therefore a potential source of resistance against wheat stem rust in barley.
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影响因子：
3.7
作者：
Engler, Carola;Kandzia, Romy;Marillonnet, Sylvestre
通讯作者：
Marillonnet, Sylvestre
DOI：
10.1126/science.1236011
发表时间：
2013-08-16
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Dangl JL;Horvath DM;Staskawicz BJ
通讯作者：
Staskawicz BJ
影响因子：
2.7
作者：
Firpo, P. D. Olivera;Newcomb, M.;Jin, Y.
通讯作者：
Jin, Y.
影响因子：
5.4
作者：
Case, Austin J.;Bhavani, Sridhar;Steffenson, Brian J.
通讯作者：
Steffenson, Brian J.
影响因子：
--
作者：
DILLMACKY, R;REES, RG;PLATZ, GJ
通讯作者：
PLATZ, GJ