Pretreatment with Restriction Enzyme or Bovine Serum Albumin for Effective PCR Amplification of Epstein-Barr Virus DNA in DNA Extracted from Paraffin-Embedded Gastric Carcinoma Tissue
Pretreatment with Restriction Enzyme or Bovine Serum Albumin for Effective PCR Amplification of Epstein-Barr Virus DNA in DNA Extracted from Paraffin-Embedded Gastric Carcinoma Tissue
复制标题
限制性内切酶或牛血清白蛋白预处理可有效 PCR 扩增从石蜡包埋的胃癌组织中提取的 DNA 中的 Epstein-Barr 病毒 DNA
DOI：
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发表时间：
1998
影响因子：
9.4
通讯作者：
T. Sairenji
中科院分区：
文献类型：
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作者：
Yukio Satoh;Noriko Takasaka;Y. Hoshikawa;M. Osaki;Satoshi Ohfuji;Hisao Ito;N. Kaibara;T. Kurata;T. Sairenji
摘要EB病毒（EBV）与胃癌的关系已通过癌细胞中存在的EBV基因组进行了研究。最近，我们发现，石蜡包埋的胃癌组织中的EBV DNA的PCR检测有效后，提取的DNA与限制性内切酶，BamHI或EcoRI的预处理。在这里，我们表明，PCR扩增也增强了预处理的DNA与其他限制性内切酶或牛血清白蛋白和其他几种蛋白质。用这些蛋白质处理可以去除从石蜡块提取的DNA样品中的PCR抑制剂。
ABSTRACT An association between Epstein-Barr virus (EBV) and gastric carcinoma has been studied through the EBV genome present in the carcinoma cells. Recently, we found that EBV DNA in paraffin-embedded gastric carcinoma tissue was detected effectively by PCR after pretreatment of the extracted DNA with a restriction enzyme,BamHI or EcoRI. Here, we show that the PCR amplification was also enhanced by pretreatment of the DNA with other restriction enzymes or with bovine serum albumin and several other proteins. Treatment with these proteins may remove a PCR inhibitor(s) in the DNA samples extracted from the paraffin blocks.
通过聚合酶链式反应检测福尔马林固定、石蜡包埋的肝组织中的乙型肝炎病毒 DNA。
DOI：
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发表时间：
1990
期刊：
The American journal of pathology
影响因子：
--
作者：
Lampertico,P;Malter,JS;Colombo,M;Gerber,MA
通讯作者：
Gerber,MA
DOI：
10.1016/0006-291x(85)90390-0
发表时间：
1985-01-01
影响因子：
3.1
作者：
GOELZ, SE;HAMILTON, SR;VOGELSTEIN, B
通讯作者：
VOGELSTEIN, B