Diagnosis and Pathogenesis of Nairobi Sheep Disease Orthonairovirus Infections in Sheep and Cattle.
Diagnosis and Pathogenesis of Nairobi Sheep Disease Orthonairovirus Infections in Sheep and Cattle.
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DOI：
10.3390/v13071250
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发表时间：
2021-06-27
期刊：
影响因子：
--
通讯作者：
Groschup MH
中科院分区：
文献类型：
--
作者：
Hartlaub J;Gutjahr B;Fast C;Mirazimi A;Keller M;Groschup MH
内罗毕羊病正鼻病毒（NSDV）是一种人畜共患蜱传虫媒病毒，可引起小型反刍动物严重胃肠炎。迄今为止，该病毒在东非和亚洲流行。然而，由于气候变化，包括传播蜱虫媒介的传播和动物运动的增加，NSDV的分布范围可能正在扩大。在该项目中，绵羊和牛（迄今为止被归类为对NSDV具有抗性）实验性感染NSDV，以比较研究物种特异性发病机制。为此，开发了几种新的诊断方法（RT-qPCR，ELISA，iIFA，mVNT，PRNT），这也将有助于未来的流行病学调查。所有攻毒绵羊（三个不同剂量组）均出现特征性临床体征、一过性病毒血症和病毒散毒-几乎与所用病毒剂量无关。一半的绵羊由于严重的临床体征（包括出血性腹泻）而被人道处死。相比之下，牛的感染过程仅为亚临床。然而，所有反刍动物表现出血清转化，这意味着，事实上，这两个物种是易感NSDV。因此，不仅羊，牛血清也可以在血清学监测计划的监测NSDV的发生和传播，在未来。
Nairobi sheep disease orthonairovirus (NSDV) is a zoonotic tick-borne arbovirus, which causes severe gastroenteritis in small ruminants. To date, the virus is prevalent in East Africa and Asia. However, due to climate change, including the spread of transmitting tick vectors and increased animal movements, it is likely that the distribution range of NSDV is enlarging. In this project, sheep and cattle (hitherto classified as resistant to NSDV) were experimentally infected with NSDV for a comparative study of the species-specific pathogenesis. For this purpose, several new diagnostic assays (RT-qPCR, ELISA, iIFA, mVNT, PRNT) were developed, which will also be useful for future epidemiological investigations. All challenged sheep (three different doses groups) developed characteristic clinical signs, transient viremia and virus shedding—almost independent on the applied virus dose. Half of the sheep had to be euthanized due to severe clinical signs, including hemorrhagic diarrhea. In contrast, the course of infection in cattle was only subclinical. However, all ruminants showed seroconversion—implying that, indeed, both species are susceptible for NSDV. Hence, not only sheep but also cattle sera can be included in serological monitoring programs for the surveillance of NSDV occurrence and spread in the future.
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DOI：
10.3390/v13030372
发表时间：
2021-02-26
期刊：
Viruses
影响因子：
--
作者：
Hartlaub J;von Arnim F;Fast C;Mirazimi A;Keller M;Groschup MH
通讯作者：
Groschup MH
影响因子：
4.5
作者：
Hartlaub J;von Arnim F;Fast C;Somova M;Mirazimi A;Groschup MH;Keller M
通讯作者：
Keller M
影响因子：
1.7
作者：
DAVIES F G;MUNGAI J N;TAYLOR M
通讯作者：
TAYLOR M
影响因子：
2.4
作者：
JESSETT, DM;DAVIES, FG;RWEYEMAMU, MM
通讯作者：
RWEYEMAMU, MM
影响因子：
1.7
作者：
EDELSTEN R M
通讯作者：
EDELSTEN R M