Experimental Challenge of Sheep and Cattle with Dugbe Orthonairovirus, a Neglected African Arbovirus Distantly Related to CCHFV.
Experimental Challenge of Sheep and Cattle with Dugbe Orthonairovirus, a Neglected African Arbovirus Distantly Related to CCHFV.
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DOI：
10.3390/v13030372
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发表时间：
2021-02-26
期刊：
影响因子：
--
通讯作者：
Groschup MH
中科院分区：
文献类型：
--
作者：
Hartlaub J;von Arnim F;Fast C;Mirazimi A;Keller M;Groschup MH
杜贝正鼻病毒（Dugbe Orthonairovirus，DUGV）是布尼亚病毒目（Bunyavirales）的一种蜱传虫媒病毒。DUGV首先在尼日利亚分离，但在另外10个非洲国家的病毒分离表明DUGV在整个非洲广泛传播。人类可能患有轻度发热性疾病，因此，DUGV被归类为生物安全水平（BSL）3级制剂。相比之下，没有疾病已被描述在动物中，虽然血清学证据存在，反刍动物是常见的宿主，并可能在这种被忽视的虫媒病毒的传播周期中发挥重要作用。在这项研究中，年轻的绵羊和小牛实验接种DUGV，以确定其易感性和研究感染的过程。此外，由于DUGV与CCHFV的亲缘关系较远，因此评估了目前可用的克里米亚-刚果出血热正鼻病毒（CCHFV）诊断检测中的潜在抗体交叉反应性。皮下接种后，所有动物均未出现临床体征或病毒血症。然而，所有反刍动物血清转化，证明了两个DUGV中和试验格式（微病毒中和试验（mVNT），空斑减少（PRNT）），通过间接免疫荧光试验和牛通过新开发的DUGV重组N蛋白ELISA。血清在商业CCHFV ELISA中不反应，而通过免疫荧光和免疫印迹测定观察到交叉反应性。
Dugbe orthonairovirus (DUGV) is a tick-borne arbovirus within the order Bunyavirales. DUGV was first isolated in Nigeria, but virus isolations in ten further African countries indicate that DUGV is widespread throughout Africa. Humans can suffer from a mild febrile illness, hence, DUGV is classified as a biosafety level (BSL) 3 agent. In contrast, no disease has been described in animals, albeit serological evidence exists that ruminants are common hosts and may play an important role in the transmission cycle of this neglected arbovirus. In this study, young sheep and calves were experimentally inoculated with DUGV in order to determine their susceptibility and to study the course of infection. Moreover, potential antibody cross-reactivities in currently available diagnostic assays for Crimean-Congo hemorrhagic fever orthonairovirus (CCHFV) were assessed as DUGV is distantly related to CCHFV. Following subcutaneous inoculation, none of the animals developed clinical signs or viremia. However, all ruminants seroconverted, as demonstrated by two DUGV neutralization test formats (micro-virus neutralization test (mVNT), plaque reduction (PRNT)), by indirect immunofluorescence assays and in bovines by a newly developed DUGV recombinant N protein ELISA. Sera did not react in commercial CCHFV ELISAs, whereas cross-reactivities were observed by immunofluorescence and immunoblot assays.
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影响因子：
4.5
作者：
Hartlaub J;von Arnim F;Fast C;Somova M;Mirazimi A;Groschup MH;Keller M
通讯作者：
Keller M
影响因子：
3.8
作者：
Gutjahr, Benjamin;Keller, Markus;Eiden, Martin
通讯作者：
Eiden, Martin
影响因子：
3.8
作者：
Mertens, Marc;Vatansever, Zati;Groschup, Martin H.
通讯作者：
Groschup, Martin H.
影响因子：
2.6
作者：
通讯作者：
--
DOI：
10.1016/s0035-9203(96)90468-x
发表时间：
1996-01-01
影响因子：
2.2
作者：
Guilherme, JM;GonellaLegall, C;Vincent, J
通讯作者：
Vincent, J