Silver-doped phosphate coacervates to inhibit pathogenic bacteria associated with wound infections: an in vitro study.
Silver-doped phosphate coacervates to inhibit pathogenic bacteria associated with wound infections: an in vitro study.
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DOI：
10.1038/s41598-022-13375-y
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发表时间：
2022-06-24
影响因子：
4.6
通讯作者：
Carta D
中科院分区：
文献类型：
--
作者：
Nikolaou A;Felipe-Sotelo M;Dorey R;Gutierrez-Merino J;Carta D
由于急性伤口或慢性伤口愈合不良，需要皮肤修复的患者有很大的需求。这些患者处于持续炎症的高风险中，经常导致危及生命的感染。因此，迫切需要能够快速刺激愈合过程并同时预防感染的新材料。基于磷酸盐的凝聚层（PC）由于其在组织再生和作为受控递送系统中的巨大潜力而受到越来越多的关注。由于是生物可吸收的，它们随时间溶解，同时以连续的方式释放治疗物质。特别令人感兴趣的是金属抗菌离子（例如Ag+）的受控释放，这是基于抗生素的常规治疗的有希望的替代方案，通常与抗菌剂抗性（AMR）相关。本研究调查了一系列的PC凝胶含有一系列浓度的抗菌离子Ag+（0.1，0.3和0.75摩尔%）。溶出度试验表明，随着时间的推移，Ag+的受控释放，导致革兰氏阳性菌和革兰氏阴性菌（金黄色葡萄球菌、粪肠球菌、大肠杆菌和铜绿假单胞菌）的非AMR和AMR菌株的细菌显著减少（高达7 log）。溶出试验还显示磷酸盐、Ca2+、Na+和Ag+的受控释放，其中大部分释放发生在前24小时。使用与人角质形成细胞（HaCaT）和细菌菌株接触的溶出产品进行的生物相容性研究表明，与对照品相比，当凝胶溶解在细胞培养基中时，细胞活力显著增加（p ≤ 0.001）。这些结果表明，凝胶状掺杂银的PC是一种很有前途的智能伤口敷料多功能材料，能够同时抑制病原菌并保持良好的细胞活力。
There is a great demand from patients requiring skin repair, as a result of poorly healed acute wounds or chronic wounds. These patients are at high risk of constant inflammation that often leads to life-threatening infections. Therefore, there is an urgent need for new materials that could rapidly stimulate the healing process and simultaneously prevent infections. Phosphate-based coacervates (PC) have been the subject of increased interest due to their great potential in tissue regeneration and as controlled delivery systems. Being bioresorbable, they dissolve over time and simultaneously release therapeutic species in a continuous manner. Of particular interest is the controlled release of metallic antibacterial ions (e.g. Ag+), a promising alternative to conventional treatments based on antibiotics, often associated with antibacterial resistance (AMR). This study investigates a series of PC gels containing a range of concentrations of the antibacterial ion Ag+ (0.1, 0.3 and 0.75 mol%). Dissolution tests have demonstrated controlled release of Ag+ over time, resulting in a significant bacterial reduction (up to 7 log), against both non-AMR and AMR strains of both Gram-positive and Gram-negative bacteria (Staphylococcus aureus, Enterococcus faecalis, Escherichia coli and Pseudomonas aeruginosa). Dissolution tests have also shown controlled release of phosphates, Ca2+, Na+ and Ag+ with most of the release occurring in the first 24 h. Biocompatibility studies, assessed using dissolution products in contact with human keratinocyte cells (HaCaT) and bacterial strains, have shown a significant increase in cell viability (p ≤ 0.001) when gels are dissolved in cell medium compared to the control. These results suggest that gel-like silver doped PCs are promising multifunctional materials for smart wound dressings, being capable of simultaneously inhibit pathogenic bacteria and maintain good cell viability.
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