Function of SREBP1 in the milk fat synthesis of dairy cow mammary epithelial cells.
Function of SREBP1 in the milk fat synthesis of dairy cow mammary epithelial cells.
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DOI：
10.3390/ijms150916998
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发表时间：
2014-09-23
影响因子：
5.6
通讯作者：
Gao XJ
中科院分区：
文献类型：
--
作者：
Li N;Zhao F;Wei C;Liang M;Zhang N;Wang C;Li QZ;Gao XJ
固醇调节元件结合蛋白（SREBPs）属于核转录因子家族。在奶牛乳腺上皮细胞（DCMECs）中，SREBPs和其他核转录因子（如过氧化物酶体增殖物激活受体γ（PPARγ））中，哪一个是最重要的正调控因子，这一问题一直存在争议。最近的研究发现，mTORC 1（雷帕霉素C1的哺乳动物靶标）调节SREBP 1以促进脂肪合成。然而，到目前为止，SREBP1和mTOR（雷帕霉素的哺乳动物靶标）途径在调节乳脂合成中的相互作用仍然知之甚少。本研究旨在鉴定SREBP1在乳脂肪合成中的功能，并表征SREBP1与DCMCs中mTOR之间的关系。采用免疫组化、Western blotting、实时定量PCR、脂滴染色和甘油三酯含量检测试剂盒，研究SREBP1过表达和基因沉默对乳脂肪合成的影响，以及硬脂酸和血清对SREBP1表达上调乳脂肪合成的影响。SREBP1被发现是乳脂合成的正调节剂，并被证明是由硬脂酸和血清调节。这些发现表明SREBP1是乳脂合成中的关键正调控因子。
Sterol regulatory element-binding proteins (SREBPs) belong to a family of nuclear transcription factors. The question of which is the most important positive regulator in milk fat synthesis in dairy cow mammary epithelial cells (DCMECs) between SREBPs or other nuclear transcription factors, such as peroxisome proliferator-activated receptor γ (PPARγ), remains a controversial one. Recent studies have found that mTORC1 (the mammalian target of rapamycin C1) regulates SREBP1 to promote fat synthesis. Thus far, however, the interaction between the SREBP1 and mTOR (the mammalian target of rapamycin) pathways in the regulation of milk fat synthesis remains poorly understood. This study aimed to identify the function of SREBP1 in milk fat synthesis and to characterize the relationship between SREBP1 and mTOR in DCMECs. The effects of SREBP1 overexpression and gene silencing on milk fat synthesis and the effects of stearic acid and serum on SREBP1 expression in the upregulation of milk fat synthesis were investigated in DCMECs using immunostaining, Western blotting, real-time quantitative PCR, lipid droplet staining, and detection kits for triglyceride content. SREBP1 was found to be a positive regulator of milk fat synthesis and was shown to be regulated by stearic acid and serum. These findings indicate that SREBP1 is the key positive regulator in milk fat synthesis.
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DOI：
10.1073/pnas.0914798107
发表时间：
2010-02-23
影响因子：
11.1
作者：
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通讯作者：
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DOI：
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影响因子：
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