Protocol for measuring mitochondrial size in mouse and human liver tissues.
Protocol for measuring mitochondrial size in mouse and human liver tissues.
复制标题
DOI：
10.1016/j.xpro.2024.102842
复制
发表时间：
2024-03-15
期刊：
影响因子：
--
通讯作者：
He, Ling
中科院分区：
文献类型：
--
作者：
Ramatchandirin, Balamurugan;Iijima, Miho;Ikeda, Arisa;Pearah, Alexia;Radovick, Sally;Wondisford, Fredric E.;Sesaki, Hiromi;He, Ling
线粒体动态过程对细胞活力、代谢活性和线粒体健康具有重要意义。在这里，我们提出了一种通过免疫荧光染色、共聚焦成像和ImageJ分析来测量线粒体大小的方案。我们描述了组织处理的步骤，抗原检索，线粒体染色使用整合免疫荧光测定和计算机图像分析来测量小鼠和人类肝脏组织中的每个线粒体大小。该方案减少了组织样品体积和处理时间为原代细胞的制备。有关该协议的使用和执行的完整细节，请参阅Pearah等人。使用免疫荧光法测定线粒体染色的详细步骤使用高压锅进行抗原提取以获得高质量的图像使用ImageJ软件定量个体线粒体大小出版商注：进行任何实验方案都需要遵守当地机构的实验室安全和伦理指导方针。线粒体动态过程对细胞活力、代谢活性和线粒体健康具有重要意义。在这里，我们提出了一种通过免疫荧光染色、共聚焦成像和ImageJ分析来测量线粒体大小的方案。我们描述了组织处理的步骤，抗原检索，线粒体染色使用整合免疫荧光测定和计算机图像分析来测量小鼠和人类肝脏组织中的每个线粒体大小。该方案减少了组织样品体积和处理时间为原代细胞的制备。
Mitochondrial dynamic process is important for cell viability, metabolic activity, and mitochondria health. Here, we present a protocol for measuring mitochondrial size through immunofluorescence staining, confocal imaging, and analysis in ImageJ. We describe the steps for tissue processing, antigen retrieval, mitochondrial staining using an integrating immunofluorescence assay, and computerized image analysis to measure each mitochondrial size in mouse and human liver tissues. This protocol reduces tissue sample volume and processing time for the preparation of primary cells. For complete details on the use and execution of this protocol, please refer to Pearah et al. Protocol for measuring mitochondrial size in mouse and human liver tissues Detailed step for mitochondrial staining using immunofluorescence assays Use high-pressure cooker for antigen retrieval to achieve high-quality images Quantify individual mitochondrial size using ImageJ software Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Mitochondrial dynamic process is important for cell viability, metabolic activity, and mitochondria health. Here, we present a protocol for measuring mitochondrial size through immunofluorescence staining, confocal imaging, and analysis in ImageJ. We describe the steps of tissue processing, antigen retrieval, mitochondrial staining using an integrating immunofluorescence assay, and computerized image analysis to measure each mitochondrial size in mouse and human liver tissues. This protocol reduces tissue sample volume and processing time for the preparation of primary cells.
登录
查看更多内容
影响因子：
5.8
作者：
Yamada T;Murata D;Kleiner DE;Anders R;Rosenberg AZ;Kaplan J;Hamilton JP;Aghajan M;Levi M;Wang NY;Dawson TM;Yanagawa T;Powers AF;Iijima M;Sesaki H
通讯作者：
Sesaki H
影响因子：
13.5
作者：
Sastre, J;Pallardo, FV;Vina, J
通讯作者：
Vina, J
影响因子：
4.6
作者：
Shami GJ;Cheng D;Verhaegh P;Koek G;Wisse E;Braet F
通讯作者：
Braet F
影响因子：
8.6
作者：
Pearah，Alexia;Ramatchandirin，Balamurugan;He，Ling
通讯作者：
He，Ling
影响因子：
29
作者：
Liesa M;Shirihai OS
通讯作者：
Shirihai OS