Sexual development genes of Neurospora crassa.
Sexual development genes of Neurospora crassa.
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粗糙脉孢菌的性发育基因。
DOI：
10.1093/genetics/132.1.149
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发表时间：
1992
期刊：
影响因子：
3.3
通讯作者：
Metzenberg,RL
中科院分区：
文献类型：
--
作者：
Nelson,MA;Metzenberg,RL
丝状真菌粗糙脉孢菌经历了复杂的有性发育程序，形成由几种特殊组织组成的子实体。使用消减杂交来分离在该性阶段优先表达的基因。许多这样的性发育 (sdv) 基因在脉孢菌基因组 DNA 的粘粒文库中被鉴定出来。亚克隆了 14 个 sdv 基因，并检查了它们在突变株中以及在杂交和营养生长条件下的表达。在营养（高氮）条件下生长的菌株中，所有受调节的转录本的丰度较低（并且在许多情况下无法检测到），这表明它们的合成需要氮饥饿。即使在杂交生长条件下，大多数 sdv 基因的表达也需要功能性 A 交配型产物，这表明该产物在性发育中起着主控作用。为了确定 sdv 基因的产物是否在性周期中发挥重要作用，使用反向遗传方法（基于 RIP（重复诱导点突变）介导的基因破坏）在基因中产生突变。分离出具有隐性交叉缺陷（显然是由 RIP 过程引起）的突变株（asd-1）；在该菌株中，早期发育是正常的，并且可能形成子囊，但从未描绘出子囊孢子。第二种隐性突变菌株（asd-2）显然是通过将转化DNA异位整合到性过程所需的基因中而产生的。在该菌株中，有性过程在早期就被阻断，子囊组织几乎没有发育。
The filamentous fungus Neurospora crassa undergoes a complex program of sexual development to form a fruiting body composed of several kinds of specialized tissue. Subtractive hybridization was used to isolate genes that are expressed preferentially during this sexual phase. Many such sexual development (sdv) genes were identified in a cosmid library of Neurospora genomic DNA. Fourteen of the sdv genes were subcloned, and their expression in mutant strains and under crossing and vegetative growth conditions was examined. All of the regulated transcripts were less abundant (and in many cases not detectable) in strains grown under vegetative (high nitrogen) conditions, suggesting that nitrogen starvation is required for their synthesis. The expression of most of the sdv genes also required a functional A mating type product, even under crossing growth conditions, suggesting that this product functions as a master control in sexual development. To determine if the products of the sdv genes play essential roles in the sexual cycle, a reverse-genetic approach (based on RIP (repeat-induced point mutation)-mediated gene disruptions) was used to create mutations in the genes. A mutant strain (asd-1) with a recessive crossing defect (apparently caused by the RIP process) was isolated; in this strain, early development is normal and may asci are formed, but ascospores are never delineated. A second recessive mutant strain (asd-2) was apparently created by ectopic integration of the transforming DNA into a gene required for the sexual process; in this strain the sexual process was blocked at an early stage, and the ascogeneous tissue underwent little development.
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制备脉孢菌 DNA：一些改进。
DOI：
10.4148/1941-4765.1643
发表时间：
1982
期刊：
Fungal Genetics Reports
影响因子：
--
作者：
J. N. Stevens;R. L. Metzenberg
通讯作者：
R. L. Metzenberg
影响因子：
56.9
作者：
CAMBARERI, EB;JENSEN, BC;SELKER, EU
通讯作者：
SELKER, EU
特定蛋白质-DNA 相互作用的体内足迹。
影响因子：
--
作者：
P. Jackson;G. Felsenfeld
通讯作者：
G. Felsenfeld
粗糙脉孢菌孢子颜色对泛酸的要求。
DOI：
10.1139/g65-022
发表时间：
1965
期刊：
Canadian journal of genetics and cytology. Journal canadien de genetique et de cytologie
影响因子：
--
作者：
S. F. Threlkeld
通讯作者：
S. F. Threlkeld
用于修饰和标记 DNA 和 RNA 的酶。
影响因子：
--
作者：
F. Cobianchi;S. H. Wilson
通讯作者：
S. H. Wilson