XRCC1 and XPD polymorphisms and their relation to the clinical course in hepatocarcinoma patients.
XRCC1 and XPD polymorphisms and their relation to the clinical course in hepatocarcinoma patients.
复制标题
DOI：
10.3892/ol.2017.6522
复制
发表时间：
2017-09
期刊：
影响因子：
2.9
通讯作者：
Zhi X
中科院分区：
文献类型：
--
作者：
Guan Q;Chen Z;Chen Q;Zhi X
本研究对肝细胞癌（HCC）患者进行基因分型，检测X射线修复交叉互补基因1（XRCC 1）和着色性干皮病互补基因D（XPD）的多态性，并分析其与HCC临床特征的关系。选择2010年1月至2011年9月在山东大学齐鲁医院住院的原发性肝癌患者172例。所有患者均行肝细胞癌切除术，术后均未发现肿瘤转移，采集患者外周静脉血3-5 ml，提取基因组DNA。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）和基因测序进行基因分型。随访5年，观察并比较不同XRCC 1和XPD基因型患者的生存情况。采用Logistic回归分析XRCC 1和XPD基因单核苷酸多态性与肝癌患者预后的关系。χ2检验显示XRCC 1 -194、XRCC 1 -280和XPD-312基因多态性与肿瘤的数目、部位和直径显著相关（p<0.05）。XRCC 1 -194位点不同基因型患者的生存曲线差异无统计学意义（p>0.05）。XRCC 1 -280位点AA和GG基因型患者的生存曲线与XPD-312位点AA、GA和GG基因型患者的生存曲线差异有统计学意义（p<0.05）。Logistic回归分析显示，XRCC 1 -194基因型不是HCC死亡的独立危险因素（p>0.05），而XRCC 1 -280（OR=1.815，p<0.01）和XPD-312（OR=1.815，p<0.01）基因型是HCC预后不良的独立危险因素。综上所述，我们的研究结果表明XRCC 1和XPD基因多态性与HCC的临床特征相关，使其成为HCC的合适预后标志物。
In this study genotyping of hepatocellular carcinoma (HCC) patients was conducted to detect polymorphisms on the X-ray repair cross-complementing 1 (XRCC1) and xeroderma pigmentosum complementary group D (XPD) genes and analyze the relationship of their presence with the clinical features of the cancer. A total of 172 patients with HCC were selected in Qilu Hospital, Shandong University, from January 2010 to September 2011. All patients underwent resection of HCC and no tumor metastases were found. Peripheral venous blood samples (3–5 ml) were collected from the patients to extract genomic DNA. Genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and gene sequencing. During the five-year follow-up, the survival of patients with various genotypes of XRCC1 and XPD genes were observed and compared. Logistic regression analysis was used to analyze the association between single nucleotide polymorphisms of XRCC1 and XPD genes and the prognosis of patients with HCC. χ2 tests showed that XRCC1-194, XRCC1-280 and XPD-312 gene polymorphisms were significantly correlated with the number, location and diameter of the tumors (p<0.05). No significant difference was found in the survival curve of patients presenting different genotypes of the XRCC1-194 locus (p>0.05). Nevertheless, a significant difference was found in the survival curve of patients with AA and GG genotypes of the XRCC1-280 locus and in the patients with AA, GA and GG genotypes of the XPD-312 locus (p<0.05). Logistic regression analysis showed that the XRCC1-194 genotype was not an independent risk factor for HCC mortality risk (p>0.05), but XRCC1-280 (OR=1.815, p<0.01) and XPD-312 (OR=1.815, p<0.01) genotypes were independent risk factors for a poor prognosis. Taken together our results point to polymorphisms in XRCC1 and XPD genes as being related to the clinical characteristics of HCC, making them suitable prognostic markers of HCC.
登录
查看更多内容
DOI：
10.1111/phpp.12108
发表时间：
2014-04
期刊：
Photodermatology, photoimmunology & photomedicine
影响因子：
--
作者：
Tamura D;DiGiovanna JJ;Khan SG;Kraemer KH
通讯作者：
Kraemer KH
影响因子：
1.6
作者：
Hung HH;Chao Y;Chiou YY;Li CP;Lee RC;Huo TI;Huang YH;Chau GY;Su CW;Yeh YC;Lin HC;Lee SD;Wu JC
通讯作者：
Wu JC
影响因子：
3.7
作者：
Petta S;Miele L;Bugianesi E;Cammà C;Rosso C;Boccia S;Cabibi D;Di Marco V;Grimaudo S;Grieco A;Pipitone RM;Marchesini G;Craxì A
通讯作者：
Craxì A
DOI：
10.3350/kjhep.2012.18.1.48
发表时间：
2012-03-01
期刊：
The Korean journal of hepatology
影响因子：
--
作者：
Lee, Sang Seok;Shin, Hyun Sung;Song, Il Han
通讯作者：
Song, Il Han
影响因子：
29.4
作者：
Segui, Nuria;Mina, Leonardo B.;Valle, Laura
通讯作者：
Valle, Laura