scm(6)A-seq reveals single-cell landscapes of the dynamic m(6)A during oocyte maturation and early embryonic development.
scm(6)A-seq reveals single-cell landscapes of the dynamic m(6)A during oocyte maturation and early embryonic development.
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scm6A-seq 揭示了卵母细胞成熟和早期胚胎发育过程中动态 m6A 的单细胞景观
DOI：
10.1038/s41467-023-35958-7
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发表时间：
2023-01-19
影响因子：
16.6
通讯作者：
Yang, Yun-Gui
中科院分区：
文献类型：
--
作者：
Yao, Huan;Gao, Chun-Chun;Zhang, Danru;Xu, Jiawei;Song, Gege;Fan, Xiu;Liang, Dao-Bo;Chen, Yu-Sheng;Li, Qian;Guo, Yanjie;Cai, Yu-Ting;Hu, Lulu;Zhao, Yong-Liang;Sun, Ying-Pu;Yang, Ying;Han, Jianyong;Yang, Yun-Gui
N6-甲基腺苷（m6 A）已被证明可以调节RNA代谢和各种生物学过程，包括配子发生和胚胎发生。然而，景观和功能的m6 A在单细胞分辨率尚未广泛研究哺乳动物卵母细胞或植入前。在这项研究中，我们开发了一种单细胞m6 A测序（scm 6A-seq）方法，同时分析了卵裂期胚胎单个卵母细胞/卵裂球中的m6 A甲基化组和转录组。我们发现，m6 A缺陷导致异常的RNA清除和随之而来的Mettl 3Gdf 9条件敲除（cKO）卵母细胞的低质量。我们进一步发现，m6 A调节的翻译和稳定性的修饰RNA在中期II（MII）卵母细胞和卵母细胞到胚胎的过渡，分别。此外，我们还观察到两细胞胚胎卵裂球之间的表观转录组中存在依赖于m6 A的不对称性。因此，scm 6A-seq允许深入研究m6 A的特征和功能，这些发现为描绘配子发生和早期胚胎发育的潜在机制提供了宝贵的单细胞分辨率资源。
N6-methyladenosine (m6A) has been demonstrated to regulate RNA metabolism and various biological processes, including gametogenesis and embryogenesis. However, the landscape and function of m6A at single cell resolution have not been extensively studied in mammalian oocytes or during pre-implantation. In this study, we developed a single-cell m6A sequencing (scm6A-seq) method to simultaneously profile the m6A methylome and transcriptome in single oocytes/blastomeres of cleavage-stage embryos. We found that m6A deficiency leads to aberrant RNA clearance and consequent low quality ofMettl3Gdf9conditional knockout (cKO) oocytes. We further revealed that m6A regulates the translation and stability of modified RNAs in metaphase II (MII) oocytes and during oocyte-to-embryo transition, respectively. Moreover, we observed m6A-dependent asymmetries in the epi-transcriptome between the blastomeres of two-cell embryo. scm6A-seq thus allows in-depth investigation into m6A characteristics and functions, and the findings provide invaluable single-cell resolution resources for delineating the underlying mechanism for gametogenesis and early embryonic development.
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YTHDF2 通过直接招募 CCR4-NOT 去腺苷酶复合物来破坏含有 m(6)A 的 RNA 的稳定性。
影响因子：
16.6
作者：
Du, Hao;Zhao, Ya;He, Jinqiu;Zhang, Yao;Xi, Hairui;Liu, Mofang;Ma, Jinbiao;Wu, Ligang
通讯作者：
Wu, Ligang
影响因子：
7.7
作者：
Cicconet, Marcelo;Gutwein, Michelle;Gunsalus, Kristin C.;Geiger, Davi
通讯作者：
Geiger, Davi
MTOR 卵母细胞阶段特异性影响决定小鼠颗粒细胞命运和卵母细胞质量
DOI：
10.1073/pnas.1800352115
发表时间：
2018-06-05
影响因子：
11.1
作者：
Guo J;Zhang T;Guo Y;Sun T;Li H;Zhang X;Yin H;Cao G;Yin Y;Wang H;Shi L;Guo X;Sha J;Eppig JJ;Su YQ
通讯作者：
Su YQ
影响因子：
14.9
作者：
Boccaletto P;Stefaniak F;Ray A;Cappannini A;Mukherjee S;Purta E;Kurkowska M;Shirvanizadeh N;Destefanis E;Groza P;Avşar G;Romitelli A;Pir P;Dassi E;Conticello SG;Aguilo F;Bujnicki JM
通讯作者：
Bujnicki JM
影响因子：
8.8
作者：
Conway AE;Van Nostrand EL;Pratt GA;Aigner S;Wilbert ML;Sundararaman B;Freese P;Lambert NJ;Sathe S;Liang TY;Essex A;Landais S;Burge CB;Jones DL;Yeo GW
通讯作者：
Yeo GW