Label free cell-tracking and division detection based on 2D time-lapse images for lineage analysis of early embryo development.
Label free cell-tracking and division detection based on 2D time-lapse images for lineage analysis of early embryo development.
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DOI:
10.1016/j.compbiomed.2014.04.011
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发表时间:
2014-08
影响因子:
7.7
通讯作者:
Geiger, Davi
中科院分区:
文献类型:
--
作者:
Cicconet, Marcelo;Gutwein, Michelle;Gunsalus, Kristin C.;Geiger, Davi
In this paper we report a database and a series of techniques related to the problem of tracking cells, and detecting their divisions, in time-lapse movies of mammalian embryos. Our contributions are: (1) a method for counting embryos in a well, and cropping each individual embryo across frames, to create individual movies for cell tracking; (2) a semi-automated method for cell tracking that works up to the 8-cell stage, along with a software implementation available to the public (this software was used to build the reported database); (3) an algorithm for automatic tracking up to the 4-cell stage, based on histograms of mirror symmetry coefficients captured using wavelets; (4) a cell-tracking database containing 100 annotated examples of mammalian embryos up to the 8-cell stage; (5) statistical analysis of various timing distributions obtained from those examples.
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