Local translation of TC10 is required for membrane expansion during axon outgrowth.
Local translation of TC10 is required for membrane expansion during axon outgrowth.
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DOI：
10.1038/ncomms4506
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发表时间：
2014-03-25
影响因子：
16.6
通讯作者：
Hengst, Ulrich
中科院分区：
文献类型：
--
作者：
Gracias, Neilia G.;Shirkey-Son, Nicole J.;Hengst, Ulrich
发育中的轴突表面在由外囊复合物介导的过程中扩张。外囊的时空调节只是部分了解。在这里，我们报告说，刺激膜扩大背根神经节（DRG）轴突是由轴突内合成的TC 10，一个小的GT 10所需的外囊功能。诱导膜扩张和轴突生长的抑制后，轴突特异性敲低TC 10 mRNA。为了确定轴突内TC 10合成与先前描述的刺激诱导的翻译的细胞骨架调节器Par 3的关系，我们调查的信号通路控制其本地翻译响应于NGF。Rheb-mTOR通路的磷酸肌醇3-激酶（PI 3 K）依赖性激活触发TC 10和Par 3的同时局部合成。这些结果揭示了膜动力学控制中的本地翻译的重要性，并表明本地化的mTOR依赖性蛋白质合成触发并行途径的同时激活。
The surface of developing axons expands in a process mediated by the exocyst complex. The spatial-temporal regulation of the exocyst is only partially understood. Here we report that stimulated membrane enlargement in dorsal root ganglion (DRG) axons is triggered by intra-axonal synthesis of TC10, a small GTPase required for exocyst function. Induced membrane expansion and axon outgrowth are inhibited after axon-specific knockdown of TC10 mRNA. To determine the relationship of intra-axonal TC10 synthesis with the previously described stimulus-induced translation of the cytoskeletal regulator Par3, we investigate the signaling pathways controlling their local translation in response to NGF. Phosphoinositide 3-kinase (PI3K)-dependent activation of the Rheb-mTOR pathway triggers the simultaneous local synthesis of TC10 and Par3. These results reveal the importance of local translation in the control of membrane dynamics and demonstrate that localized, mTOR-dependent protein synthesis triggers the simultaneous activation of parallel pathways.
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DOI：
10.1523/jneurosci.3907-09.2009
发表时间：
2009-10-21
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Dupraz S;Grassi D;Bernis ME;Sosa L;Bisbal M;Gastaldi L;Jausoro I;Cáceres A;Pfenninger KH;Quiroga S
通讯作者：
Quiroga S
影响因子：
4.8
作者：
Castro, AF;Rebhun, JF;Quilliam, LA
通讯作者：
Quilliam, LA
影响因子：
4.5
作者：
Gumy, Laura F.;Yeo, Giles S. H.;Fawcett, James W.
通讯作者：
Fawcett, James W.
DOI：
10.1111/j.1600-0854.2012.01353.x
发表时间：
2012-07
期刊：
Traffic (Copenhagen, Denmark)
影响因子：
--
作者：
Heider MR;Munson M
通讯作者：
Munson M
影响因子：
21.3
作者：
Hengst, Ulrich;Deglincerti, Alessia;Kim, Hyung Joon;Jeon, Noo Li;Jaffrey, Samie R.
通讯作者：
Jaffrey, Samie R.