Early Steroid-Induced Osteonecrosis of Rabbit Femoral Head and Panax notoginseng Saponins: Mechanism and Protective Effects.
Early Steroid-Induced Osteonecrosis of Rabbit Femoral Head and Panax notoginseng Saponins: Mechanism and Protective Effects.
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兔股骨头和三七皂苷早期类固醇引起的骨坏死：机制和保护作用。
DOI：
10.1155/2015/719370
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Zhang C
中科院分区：
文献类型：
--
作者：
Qiang H;Liu H;Ling M;Wang K;Zhang C
背景本研究旨在探讨氧化应激在激素性股骨头缺血性坏死（SANFH）中的发病机制及三七总皂苷（PNS）对早期SANFH的保护作用。方法.将80只成年新西兰大白兔随机分为对照组、模型组和三七总皂苷组。模型组耳缘静脉注射马血清，然后臀肌注射甲基强的松龙。PNS组在甲基强的松龙治疗前应用PNS连续14天。于不同时间点取血清和股骨头，测定血清总抗氧化能力（T-AOC）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-PX）、羟自由基（·OH）和丙二醛（MDA）含量。激素治疗后2周，所有股骨头进行MRI和HE染色。结果模型组出现典型的早期骨坏死症状。结果表明，三七总皂甙能明显改善T-AOC水平的下降，提高SOD和GSH-PX活性，抑制·OH能力，升高MDA水平。三七总皂苷能改善股骨头的MRI及病理改变，明显降低股骨头坏死的发生率。结论我们的研究发现，氧化应激在SANFH的发生中起着积极的作用，其中活性氧是SANFH的直接原因。三七总皂苷对激素性股骨头坏死早期有保护作用，其机制可能与其抗氧化作用有关。
Background. This study was aimed at investigating the pathogenesis of oxidative stress in steroid-induced avascular necrosis of the femoral head (SANFH) and at exploring the mechanism and protective effects of Panax notoginseng saponins (PNS) on early SANFH. Methods. 80 adult New Zealand rabbits were randomly divided into control group, model group, and PNS group. In model group, equine serum was injected into auricular vein; then methylprednisolone was injected into gluteus. In PNS group, PNS was applied for 14 consecutive days before methylprednisolone management. At different time points, serum and femoral heads were prepared for T-AOC, SOD, GSH-PX, ·OH, and MDA determination. Two weeks after steroid management, all femoral heads were assessed with MRI and HE staining. Results. Typical early osteonecrosis symptoms were observed in model group. Our results showed that PNS could significantly ameliorate the decrease of T-AOC level, improve SOD and GSH-PX activity, suppress ·OH ability, and augment MDA level. Besides, PNS improved MRI and pathological changes of the femoral head, markedly reducing the incidence of osteonecrosis. Conclusion. Based on our research, we found oxidative stress played a positive role in the occurrence of SANFH where reactive oxygen species was the direct cause. PNS could protect rabbits against early steroid-induced osteonecrosis of femoral head by its antioxidative effect.
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