Identification of melanoma antigens that are immunogenic in humans and expressed in vivo.
Identification of melanoma antigens that are immunogenic in humans and expressed in vivo.
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鉴定在人类中具有免疫原性并在体内表达的黑色素瘤抗原。
DOI：
10.1093/jnci/90.2.146
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Bystryn,JC
中科院分区：
文献类型：
--
作者：
Applebaum,J;Reynolds,S;Knispel,J;Oratz,R;Shapiro,R;Bystryn,JC
Background.In the development of an antimelanoma vaccine, a critical factor is the identification of antigens that induce a strong immune response in humans and that are expressed by melanoma cellsin vivo. The aim of this study was to identify candidate antigens for such vaccine.Methods:Sixtynine patients with surgically resected melanomas (American Joint Commission on Cancer [AJCC] stage III) were immunized with a polyvalent vaccine containing multiple melanoma antigens. Antimelanoma antibodies generated in the patients' sera were used as probes to identify the melanoma antigens that are immunogenic in humans and that are expressed on the tumor tissuein vivo. Such responses were determined by an immunoblotting assay that employed an antigen source prepared from membrane fractions of freshly excised melanoma tissue.Results and Conclusions: Vaccine treatment stimulated antibody responses in 35 (51%; 95% confidence interval [CI] = 39%–63%) of 69 sequentially enrolled patients. The antibodies were directed to one or more antigens with molecular masses of 45, 59, 68, 79, 89, 95, and/or 110 kd. The most immunogenic antigens were p110 and p68, which induced responses in 33% (95% CI = 22%∓44%) and 25% (95% CI = 15%-35%) of patients, respectively. Both antigens were commonly expressed on different melanomas, but they were absent on autologous normal tissue and on an unrelated allogeneic tumor. All the above antigens are attractive candidates for vaccine construction. [J Nall Cancer Inst 1998;90: 146-9]
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DOI：
10.1084/jem.179.3.1005
发表时间：
1994-03-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Bakker AB;Schreurs MW;de Boer AJ;Kawakami Y;Rosenberg SA;Adema GJ;Figdor CG
通讯作者：
Figdor CG
在用痘苗病毒黑色素瘤溶瘤物免疫的患者中诱导针对 M(r) 31,000 黑色素瘤抗原的 IgG 抗体。
影响因子：
11.2
作者：
O. Berthier‐Vergnes;J. Portoukalian;É. Leftheriotis;J. Doré
通讯作者：
J. Doré
人单克隆抗体在人黑色素瘤的 M(r) 43,000 蛋白中鉴定出免疫反应性四肽序列 (Lys-Tyr-Gln-Ile)。
影响因子：
11.2
作者：
Oka,T;Kikumoto,Y;Itakura,K;Morton,DL;Irie,RF
通讯作者：
Irie,RF
影响因子：
5.4
作者：
WOLFEL, T;VANPEL, A;BOON, T
通讯作者：
BOON, T
多价黑色素瘤疫苗中免疫原性人类黑色素瘤抗原的鉴定。
影响因子：
11.2
作者：
Bystryn,JC;Henn,M;Li,J;Shroba,S
通讯作者：
Shroba,S