Calcium-induced alteration of mitochondrial morphology and mitochondrial-endoplasmic reticulum contacts in rat brown adipocytes.
Calcium-induced alteration of mitochondrial morphology and mitochondrial-endoplasmic reticulum contacts in rat brown adipocytes.
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DOI：
10.4081/ejh.2014.2377
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发表时间：
2014-09-09
期刊：
影响因子：
--
通讯作者：
Korac A
中科院分区：
文献类型：
--
作者：
Golic I;Velickovic K;Markelic M;Stancic A;Jankovic A;Vucetic M;Otasevic V;Buzadzic B;Korac B;Korac A
线粒体是维持细胞生物能量和完整性的关键细胞器，其对[Ca 2 +]i稳态的调节已在许多细胞类型中进行了研究。我们使用成像和分子生物学技术研究了Ca-SANDOZ®对棕色脂肪细胞线粒体的短期处理。将两个月大的雄性Wistar大鼠分为两组：饮用Ca-SANDOZ®或饮用自来水（对照）三天。茜素红S染色显示治疗组大鼠棕色脂肪细胞内Ca 2+水平升高，焦锑酸钾染色显示细胞质、线粒体和脂滴周围电子致密区。Ca-SANDOZ®减少线粒体数量，但增加其大小和线粒体嵴体积。透射电子显微镜检查显示，与对照组相比，Ca-SANDOZ®处理组中有许多放大的融合样线粒体，并且在一些棕色脂肪细胞中有巨线粒体。Ca 2+饮食影响线粒体融合，线粒体融合蛋白1（MFN 1）和线粒体融合蛋白2（MFN 2）增加，线粒体分裂，动力蛋白相关蛋白1（DRP 1）减少。共聚焦显微镜显示功能线粒体和内质网（ER）之间的共定位率较高。解偶联蛋白1（UCP 1）的表达水平升高，免疫组化和Western blot分析证实了这一点。这些结果表明，Ca-SANDOZ®刺激线粒体融合，增加细胞-ER接触和棕色脂肪细胞的产热能力。
Mitochondria are key organelles maintaining cellular bioenergetics and integrity, and their regulation of [Ca2+]i homeostasis has been investigated in many cell types. We investigated the short-term Ca-SANDOZ® treatment on brown adipocyte mitochondria, using imaging and molecular biology techniques. Two-month-old male Wistar rats were divided into two groups: Ca-SANDOZ® drinking or tap water (control) drinking for three days. Alizarin Red S staining showed increased Ca2+ level in the brown adipocytes of treated rats, and potassium pyroantimonate staining localized electron-dense regions in the cytoplasm, mitochondria and around lipid droplets. Ca-SANDOZ® decreased mitochondrial number, but increased their size and mitochondrial cristae volume. Transmission electron microscopy revealed numerous enlarged and fusioned-like mitochondria in the Ca-SANDOZ® treated group compared to the control, and megamitochondria in some brown adipocytes. The Ca2+ diet affected mitochondrial fusion as mitofusin 1 (MFN1) and mitofusin 2 (MFN2) were increased, and mitochondrial fission as dynamin related protein 1 (DRP1) was decreased. Confocal microscopy showed a higher colocalization rate between functional mitochondria and endoplasmic reticulum (ER). The level of uncoupling protein-1 (UCP1) was elevated, which was confirmed by immunohistochemistry and Western blot analysis. These results suggest that Ca-SANDOZ® stimulates mitochondrial fusion, increases mitochondrial-ER contacts and the thermogenic capacity of brown adipocytes.
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影响因子：
64.5
作者：
Cárdenas C;Miller RA;Smith I;Bui T;Molgó J;Müller M;Vais H;Cheung KH;Yang J;Parker I;Thompson CB;Birnbaum MJ;Hallows KR;Foskett JK
通讯作者：
Foskett JK
影响因子：
4
作者：
Hayato, Ryotaro;Higure, Yoko;Kuba, Kenji
通讯作者：
Kuba, Kenji
DOI：
10.1083/jcb.200211046
发表时间：
2003-01-20
期刊：
The Journal of cell biology
影响因子：
--
作者：
Chen H;Detmer SA;Ewald AJ;Griffin EE;Fraser SE;Chan DC
通讯作者：
Chan DC
影响因子：
64.5
作者：
HAJNOCZKY, G;ROBBGASPERS, LD;THOMAS, AP
通讯作者：
THOMAS, AP
DOI：
10.1111/j.1432-1033.1984.tb08173.x
发表时间：
1984-01-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
CONNOLLY, E;NANBERG, E;NEDERGAARD, J
通讯作者：
NEDERGAARD, J