Suppression of Inflammation Delays Hair Cell Regeneration and Functional Recovery Following Lateral Line Damage in Zebrafish Larvae.
Suppression of Inflammation Delays Hair Cell Regeneration and Functional Recovery Following Lateral Line Damage in Zebrafish Larvae.
复制标题
抑制炎症会延迟斑马鱼幼虫侧线损伤后的毛细胞再生和功能恢复
DOI：
10.3390/biom10101451
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发表时间：
2020-10-16
期刊：
影响因子：
5.5
通讯作者：
Hu B
中科院分区：
文献类型：
--
作者：
Zhang R;Liu X;Li Y;Wang M;Chen L;Hu B
背景人类耳蜗毛细胞丢失后不能再生。相比之下，鱼类和两栖动物体内的这些物质在受损后具有非凡的再生能力。以前的研究主要集中在毛细胞再生的机制上，如Wnt和Notch信号。这些研究忽略了这样一个事实，即再生的开始伴随着大量的炎症反应。这种炎症在毛细胞再生中的作用尚不清楚。此外，还没有合适的行为学方法来定量评估侧线毛细胞再生后的功能恢复情况。结果抑制炎症后，幼虫侧线毛细胞的再生和血液流变性的恢复明显延缓。钙离子成像显示，在再生早期，炎症抑制组神经肥大细胞的功能弱于非炎症抑制组，后期恢复正常。钙成像也揭示了再生过程中功能和数量不匹配的原因。结论我们的结果同时表明，当毛细胞受损时，抑制炎症会延迟毛细胞的再生和功能恢复。本研究可能为如何促进成年哺乳动物毛细胞再生和功能恢复提供新的思路。
Background Human cochlear hair cells cannot regenerate after loss. In contrast, those in fish and amphibians have a remarkable ability to regenerate after damaged. Previous studies focus on mechanisms of hair cell regeneration, such as Wnt and Notch signals. These studies ignore the fact that the beginning of regeneration is accompanied by a large number of inflammatory responses. The role of this inflammation in hair cell regeneration is still unknown. In addition, there is no appropriate behavioral method to quantitatively evaluate the functional recovery of lateral line hair cells after regeneration. Results In this study, we found that when inflammation was suppressed, the regeneration of lateral line hair cells and the recovery of the rheotaxis of the larvae were significantly delayed. Calcium imaging showed that the function of the neuromasts in the inflammation-inhibited group was weaker than that in the non-inflammation-inhibited group at the Early Stage of regeneration, and returned to normal at the Late Stage. Calcium imaging also revealed the cause of the mismatch between the function and quantity during regeneration. Conclusions Our results, meanwhile, suggest that suppressing inflammation delays hair cell regeneration and functional recovery when hair cells are damaged. This study may provide a new idea for how to promote hair cell regeneration and functional recovery in adult mammals.
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影响因子：
16.2
作者：
Mizutari K;Fujioka M;Hosoya M;Bramhall N;Okano HJ;Okano H;Edge AS
通讯作者：
Edge AS
细胞凋亡引起的JNK的增殖解密激活的遗传模型，并确定了果蝇中EGFR信号传导对组织再生反应的需求。
影响因子：
4.5
作者：
Fan Y;Wang S;Hernandez J;Yenigun VB;Hertlein G;Fogarty CE;Lindblad JL;Bergmann A
通讯作者：
Bergmann A
影响因子：
2.8
作者：
Chen, L;Trautwein, PG;Salvi, RJ
通讯作者：
Salvi, RJ
DOI：
10.1111/j.1749-6632.1996.tb15693.x
发表时间：
1996-01-01
期刊：
NEW DIRECTIONS IN VESTIBULAR RESEARCH
影响因子：
--
作者：
Baird, RA;Steyger, PS;Schuff, NR
通讯作者：
Schuff, NR
影响因子：
4.1
作者：
Nesan, Dinushan;Vijayan, Mathilakath M.
通讯作者：
Vijayan, Mathilakath M.