Epidemic plasmid carrying bla(CTX-M-15) in Klebsiella penumoniae in China.
Epidemic plasmid carrying bla(CTX-M-15) in Klebsiella penumoniae in China.
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DOI：
10.1371/journal.pone.0052222
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Zhong NS
中科院分区：
文献类型：
--
作者：
Zhuo C;Li XQ;Zong ZY;Zhong NS
目的调查我国南方地区携带bla CTX-M-15的肺炎克雷伯菌的流行病学特征，分析bla CTX-M-15的遗传环境。 PCR 和 DNA 测序用于检测和表征 bla CTX-M-15 的遗传背景。通过脉冲场凝胶电泳确定携带 bla CTX-M-15 的分离株的克隆相关性。通过交配获得携带bla CTX-M-15的接合质粒，并进一步进行限制性分析和复制子分型。 2007年10月至2008年10月，从中国9家医院收集了总共47株产ESBL的肺炎克雷伯菌分离株。分离株被分为不同的克隆组。根据 S1-PFGE 和限制性分析确定，bla CTX-M-15 的局部扩散主要由一种主要接合质粒介导。属于不相容组FII的90-kb质粒是肺炎克雷伯菌中bla CTX-M-15的主要载体。除bla TEM-1外，质粒中未发现bla SHV、bla DHA-1、bla OXA-1、qnrB、qnrS、aac(3)-II、aac(6′)-Ib等抗性基因。经接合基因序列比对，与此前在中国浙江省肺炎克雷伯菌中发现的质粒pKF3-94 100%一致。 bla CTX-M-15在中国南方肺炎克雷伯菌中流行。 bla CTX-M-15 的传播似乎是由于 90 kb 流行质粒的水平转移。
To investigate the local epidemiology of Klebsiella penumoniae carrying bla CTX-M-15 in southern China and to characterize the genetic environment of bla CTX-M-15. PCR and DNA sequencing were used to detect and characterize the genetic contexts of bla CTX-M-15. The clonal relatedness of isolates carrying bla CTX-M-15 was determined by pulse-field gel electrophoresis. Conjugative plasmids carrying bla CTX-M-15 were obtained by mating and were further subject to restriction analysis and replicon typing. A total of 47CTX-M-15 ESBL-producing isolates of K. pneumoniae were collected from nine hospitals in China from October 2007 to October 2008. Isolates were clustered into various clonal groups. The local spread of bla CTX-M-15 was mainly mediated by one major conjugative plasmid as determined by S1-PFGE and restriction analysis. A 90-kb plasmid belonging to incompatible group FII was the major carrier of bla CTX-M-15 in K. pneumoniae. Except bla TEM-1, the resistance genes such as bla SHV, bla DHA-1, bla OXA-1, qnrB, qnrS, aac(3)-II, and aac(6′)-Ib were not found in the plasmid. In the comparing of conjugative gene sequence, it is 100% identical with the plasmid pKF3–94, which was found in K. pneumonia from Zhejiang province of china previously. bla CTX-M-15 was prevalent in K. pneumonia of southern China. The dissemination of bla CTX-M-15 appeared to be due to the horizontal transfer of a 90-kb epidemic plasmid.
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影响因子：
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作者：
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