A Novel Secreted Protein-Related Gene Signature Predicts Overall Survival and Is Associated With Tumor Immunity in Patients With Lung Adenocarcinoma.
A Novel Secreted Protein-Related Gene Signature Predicts Overall Survival and Is Associated With Tumor Immunity in Patients With Lung Adenocarcinoma.
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一种新的分泌蛋白相关基因特征可预测肺腺癌患者的总体生存率并与肿瘤免疫相关
DOI：
10.3389/fonc.2022.870328
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发表时间：
2022
影响因子：
4.7
通讯作者：
中科院分区：
文献类型：
--
作者：
分泌蛋白是人类蛋白质组中的重要蛋白质，约占蛋白质组的十分之一。然而，分泌蛋白相关基因在肺腺癌（LUAD）中的预后价值尚未得到全面探讨。在这项研究中，我们筛选了379个差异表达的分泌蛋白基因（DESPRGs），通过分析表达谱与LUAD患者从癌症基因组图谱数据库。在单变量考克斯回归和最小绝对收缩和选择算子方法回归分析后，选择9个预后SPRG以产生分泌蛋白相关风险评分（SPRrisk），包括CLEC 3B、C1 QTNF 6、TCN 1、F2、FETUB、IGFBP 1、ANGPTL 4、IFNE和CCL 20。Kaplan-Meier生存曲线分析和受试者工作特征曲线分析确定预后模型的预测准确性。此外，基于独立的预后因素（SPR风险和临床分期），建立了预测总生存率的准确性提高的诺模图。利用我们的临床样本和数据集，通过定量实时PCR和酶联免疫吸附试验验证DESPRGs。我们的研究结果表明，SPRrisk可以准确地预测LUAD患者的预后。高风险患者的免疫、基质和ESTIMATE评分较低，肿瘤纯度较高。较高的SPR风险也与CD 8 + T细胞和M1巨噬细胞的丰度呈负相关。此外，人类白细胞抗原家族和免疫检查点的几个基因在高SPR风险组中以低水平表达。我们的研究结果为评估个体预后和选择个性化治疗方式提供了一些见解。
Secreted proteins are important proteins in the human proteome, accounting for approximately one-tenth of the proteome. However, the prognostic value of secreted protein-related genes has not been comprehensively explored in lung adenocarcinoma (LUAD). In this study, we screened 379 differentially expressed secretory protein genes (DESPRGs) by analyzing the expression profile in patients with LUAD from The Cancer Genome Atlas database. Following univariate Cox regression and least absolute shrinkage and selection operator method regression analysis, 9 prognostic SPRGs were selected to develop secreted protein-related risk score (SPRrisk), including CLEC3B, C1QTNF6, TCN1, F2, FETUB, IGFBP1, ANGPTL4, IFNE, and CCL20. The prediction accuracy of the prognostic models was determined by Kaplan–Meier survival curve analysis and receiver operating characteristic curve analysis. Moreover, a nomogram with improved accuracy for predicting overall survival was established based on independent prognostic factors (SPRrisk and clinical stage). The DESPRGs were validated by quantitative real-time PCR and enzyme-linked immunosorbent assay by using our clinical samples and datasets. Our results demonstrated that SPRrisk can accurately predict the prognosis of patients with LUAD. Patients with a higher risk had lower immune, stromal, and ESTIMATE scores and higher tumor purity. A higher SPRrisk was also negatively associated with the abundance of CD8+ T cells and M1 macrophages. In addition, several genes of the human leukocyte antigen family and immune checkpoints were expressed in low levels in the high-SPRrisk group. Our results provided some insights into assessing individual prognosis and choosing personalized treatment modalities.
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DOI：
10.1007/978-1-4614-0887-1_12
发表时间：
2012-01-01
期刊：
ENDOCRINE FGFS AND KLOTHOS
影响因子：
--
作者：
Lin, Benjamin C.;Desnoyers, Luc R.
通讯作者：
Desnoyers, Luc R.
影响因子：
39.3
作者：
Li Y;Hou G;Zhou H;Wang Y;Tun HM;Zhu A;Zhao J;Xiao F;Lin S;Liu D;Zhou D;Mai L;Zhang L;Zhang Z;Kuang L;Guan J;Chen Q;Wen L;Zhang Y;Zhuo J;Li F;Zhuang Z;Chen Z;Luo L;Liu D;Chen C;Gan M;Zhong N;Zhao J;Ren Y;Xu Y
通讯作者：
Xu Y
DOI：
10.1073/pnas.0914019107
发表时间：
2010-02-23
影响因子：
11.1
作者：
Gonzalez, Rodolfo;Jennings, Lori L.;Lesley, Scott A.
通讯作者：
Lesley, Scott A.
影响因子：
5.8
作者：
Hubbell, E;Liu, WM;Mei, R
通讯作者：
Mei, R
影响因子：
9.3
作者：
Baratto, Lucia;Duan, Heying;Iagaru, Andrei
通讯作者：
Iagaru, Andrei