Macrophage-Like Cell Density Is Increased in Proliferative Diabetic Retinopathy Characterized by Optical Coherence Tomography Angiography.
Macrophage-Like Cell Density Is Increased in Proliferative Diabetic Retinopathy Characterized by Optical Coherence Tomography Angiography.
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DOI：
10.1167/iovs.62.10.2
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发表时间：
2021-08-02
影响因子：
4.4
通讯作者：
Lavine JA
中科院分区：
文献类型：
--
作者：
Ong JX;Nesper PL;Fawzi AA;Wang JM;Lavine JA
目的应用光学相干断层扫描血管造影（OCTA）定量分析不同严重程度糖尿病视网膜病变（DR）玻璃体视网膜界面巨噬细胞样细胞（MLCs）的特征。该研究包括72名受试者的72只眼睛：18名健康对照，22名糖尿病（DM）无DR，17名非增殖性DR（NPDR）和15名增殖性DR（PDR）。我们对每只眼睛进行重复（平均6.5次，范围3-10次）黄斑OCTA扫描。我们对玻璃体视网膜界面上方的3 µm OCT板进行配准和平均，以可视化MLC。使用半自动化的方法，我们二进制化和量化MLC和组间比较MLC密度。我们还评估了MLC相对于基础浅表毛细血管丛血管系统的分布，并量化了血管周围30 µm分水岭区域和缺血区（定义为距离最近血管>30 µm）内血管上的MLC。PDR中MLC密度是所有其他组的2.8- 3.8倍（P < 0.05）。PDR中MLC密度在血管周围区域（3.3- 4.2倍; P < 0.05 vs. all）和血管（3.0- 4.0倍; P < 0.05 vs. all）增加最多，在缺血区域增加程度较小（2.3- 3.4倍; P < 0.05 vs. all）。无DR、NPDR和PDR的糖尿病患者的MLCs更容易定位于血管上（所有患者P < 0.05），而健康眼则不然。PDR的MLC密度显著增加。MLCs在糖尿病患者的血管上聚集，而在健康眼中则没有。需要进一步的研究来确认MLCs在DR中的来源、身份和功能。
To quantitatively characterize macrophage-like cells (MLCs) at the vitreoretinal interface in different severity stages of diabetic retinopathy (DR) using optical coherence tomography angiography (OCTA). The study included 72 eyes of 72 subjects: 18 healthy controls, 22 diabetes mellitus (DM) without DR, 17 nonproliferative DR (NPDR), and 15 proliferative DR (PDR). We obtained repeated (average, 6.5; range, 3–10) macular OCTA scans for each eye. We registered and averaged the 3-µm OCT slab above the vitreoretinal interface to visualize MLCs. Using a semiautomated method, we binarized and quantified MLCs and compared MLC densities among groups. We also evaluated MLC distribution relative to underlying superficial capillary plexus vasculature and quantified MLCs overlying blood vessels within the perivascular 30-µm watershed region and within ischemic zones (defined as >30 µm from the nearest vessel). MLC density was 2.8- to 3.8-fold higher in PDR compared with all other groups (P < 0.05 for all). MLC density in PDR was most increased in perivascular areas (3.3- to 4.2-fold; P < 0.05 vs. all) and on blood vessels (3.0- to 4.0-fold; P < 0.05 vs. all), and elevated to a lesser extent in ischemic areas (2.3- to 3.4-fold; P < 0.05 vs. all). MLCs were more likely to localize on blood vessels in DM without DR, NPDR, and PDR (P < 0.05 for all), but not healthy eyes. MLC density was significantly increased in PDR. MLCs clustered on blood vessels in diabetic but not in healthy eyes. Further studies are needed to confirm the origin, identity, and function of MLCs during DR.
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