Differential Gene Expression Associated with Meningeal Injury in Acute Mild Traumatic Brain Injury.
Differential Gene Expression Associated with Meningeal Injury in Acute Mild Traumatic Brain Injury.
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与急性轻度创伤性脑损伤中脑膜损伤相关的差异基因表达。
DOI：
10.1089/neu.2016.4479
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发表时间：
2017
影响因子：
4.2
通讯作者：
Latour,LawrenceL
中科院分区：
文献类型：
--
作者：
Livingston,WhitneyS;Gill,JessicaM;Cota,MartinR;Olivera,Anlys;O'Keefe,JessicaL;Martin,Christiana;Latour,LawrenceL
创伤性脑损伤（TBI）后脑膜损伤并不罕见，但很少有研究致力于了解相关的生物学。在脑震荡事件之后，在一些患者中观察到脑膜在对比后磁共振成像（MRI）上异常增强，但不是全部。这项工作的目的是确定脑膜损伤患者中差异表达的基因。疑似TBI的急诊室患者在受伤后48小时内接受标准研究MRI和抽血。包括两组患者：在增强后MRI上有和没有脑膜异常增强的患者，两者都没有其他成像结果。使用Affytek（Santa Clara，CA）和Partek Genomics Suite（Partek，Inc.，圣路易斯，密苏里州）软件（错误发现率，<0.05）。入组了40例患者，从损伤到MRI/抽血的时间为16.8 h（四分位距，7.5-24.1）。我们观察到76个基因在脑膜损伤患者与无脑膜损伤患者相比差异表达，如伊加Fc片段受体、多个C2结构域、跨膜2和G蛋白偶联受体27，这些基因先前与启动炎症介质、吞噬作用和其他调节机制相关。对比后MRI能够检测脑膜损伤，并具有通过基因表达观察到的独特生物学特征。这些发现表明，急性炎症反应发生在脑震荡后对脑膜损伤的反应。
Injury to the meninges is not uncommon after traumatic brain injury (TBI), yet minimal research has been directed toward understanding the relevant biology. After a concussive event, the meninges are observed to abnormally enhance on post-contrast magnetic resonance imaging (MRI) in some patients, but not all. The aim of this work is to identify genes differentially expressed in patients with meningeal injury. Patients presenting to the emergency room with suspected TBI received a standard research MRI and blood draw within 48 h of injury. Two groups of patients were included: those with and without abnormal enhancement of the meninges on post-contrast MRI, both without other imaging findings. Groups were compared on microarray gene expression in peripheral blood samples using Affymetrix (Santa Clara, CA) and Partek Genomics Suite (Partek, Inc., St. Louis, MO) software (false discovery rate, <0.05). Forty patients were enrolled with a time from injury to MRI/blood draw of 16.8 h (interquartile range, 7.5–24.1). We observed 76 genes to be differentially expressed in patients with meningeal injury compared to those without, such as receptor for Fc fragment of IgA, multiple C2 domains, transmembrane 2, and G-protein-coupled receptor 27, which have been previously associated with initiating inflammatory mediators, phagocytosis, and other regulatory mechanisms. Post-contrast MRI is able to detect meningeal injury and has a unique biological signature observed through gene expression. These findings suggest that an acute inflammatory response occurs in response to injury to the meninges following a concussion.
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影响因子：
6.2
作者：
Gill, M;Windemuth, R;Green, SM
通讯作者：
Green, SM
DOI：
10.1186/cc973
发表时间：
2001
期刊：
Critical care (London, England)
影响因子：
--
作者：
Grmec S;Gasparovic V
通讯作者：
Gasparovic V
脂多糖和细胞因子增强人单核细胞 IgA 受体的表达和功能。
DOI：
--
发表时间：
1994
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Shen,L;Collins,JE;Schoenborn,MA;Maliszewski,CR
通讯作者：
Maliszewski,CR
影响因子：
4.4
作者：
Deng, GM;Liu, ZQ;Tarkowski, A
通讯作者：
Tarkowski, A
影响因子：
3.1
作者：
C. Borlongan;Loren E. Glover;P. Sanberg;D. Hess
通讯作者：
C. Borlongan;Loren E. Glover;P. Sanberg;D. Hess