An opto-thermocapillary cell micromanipulator.

An opto-thermocapillary cell micromanipulator.
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DOI:
10.1039/c3lc50389e
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发表时间:
2013-06-21
期刊:
影响因子:
6.1
通讯作者:
Ohta AT
Ohta AT
中科院分区:
工程技术1区
文献类型:
--
作者:
Hu W;Fan Q;Ohta AT

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这里介绍了一种能够进行单细胞操作和图案化的光热毛细管显微操作器(OTMm)。 OTMm 使用聚焦在 ITO 基板上的近红外激光来诱导热毛细管对流,从而能够以高达 40 pN 的力捕获和运输活细胞。 OTMm 补充了其他细胞操作技术,例如光镊和介电泳,因为它较少依赖于工作环境的光学和电学特性,并且可以在多种类型的细胞培养基中发挥作用。 OTMm 用于在两种流行的水凝胶中构建单细胞基质:PEGDA 和琼脂糖。在两种水凝胶中均观察到高存活率,并且在随后的培养过程中琼脂糖中图案化的细胞扩散和迁移。
An opto-thermocapillary micromanipulator (OTMm) capable of single-cell manipulation and patterning is presented here. The OTMm uses a near-infrared laser focused on an ITO substrate to induce thermocapillary convection that can trap and transport living cells with forces of up to 40 pN. The OTMm complements other cell-manipulation technologies such as optical tweezers and dielectrophoresis, as it is less dependent upon the optical and electrical properties of the working environment, and can function in many types of cell culture media. The OTMm was used to construct single-cell matrices in two popular hydrogels: PEGDA and agarose. High viability rates were observed in both hydrogels, and cells patterned in agarose spread and migrated during subsequent culturing.
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