Detection of Osteoarthritis Inflammation by Single-Photon Emission Computed Tomography Based on an Inflammation-Targeting Peptide cFLFLF.
Detection of Osteoarthritis Inflammation by Single-Photon Emission Computed Tomography Based on an Inflammation-Targeting Peptide cFLFLF.
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DOI：
10.1007/s11307-021-01616-x
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发表时间：
2021-12
影响因子：
3.1
通讯作者：
Cui Q
中科院分区：
文献类型：
--
作者：
Yang X;Ignozzi AJ;He R;Zhu D;Wang X;Chordia MD;Pan D;Cui Q
尽管炎症已被认为是骨关节炎（OA）发病机制的关键过程，但临床上仍然没有可以特异性诊断 OA 炎症活动的非侵入性成像方式。在本研究中，甲酰肽受体 1 (Fpr1) 靶向探针 cFLFLF-PEG-HYNIC-99mTc 和单光子发射计算机断层扫描 (SPECT) 成像用于通过靶向参与 OA 发病机制的巨噬细胞来检测炎症活动。进行体外实验来评估巨噬细胞炎症反应期间 Fpr1 的表达。在体内研究中，进行了前交叉韧带横断（ACLT）手术，并评估了磁共振成像（MRI）和组织学数据，以分析小鼠和大鼠的 OA 模型。使用放射性探针 cFLFLF-PEG-HYNIC-99mTc 和 SPECT 成像来证实 OA 相关炎症，并比较 ACLT 与假膝。体外巨噬细胞激活导致 Fpr1 表达显着增加。小鼠和大鼠的体内实验产生了类似的结果。 MRI 和组织学分析表明 ACLT 膝关节存在明显的关节退化。与假膝盖相比，ACLT 膝盖从探针产生了更强的信号。值得注意的是，ACLT/假膝信号强度的比率随着 OA 的进展而降低，表明 OA 进展早期的差异更大。放射性探针 cFLFLF-PEG-HYNIC-99mTc 和 SPECT 成像通过靶向膝关节中的 Fpr1 表达，可有效检测和监测 OA 进展期间的炎症。
Although inflammation has been recognized as a key process in the pathogenesis of osteoarthritis (OA), there remains no clinical non-invasive imaging modality that can specifically diagnose inflammatory activity of OA. In this study, a formyl peptide receptor 1 (Fpr1) targeting probe cFLFLF-PEG-HYNIC-99mTc and Single-Photon Emission Computed Tomography (SPECT) imaging was used to detect inflammatory activity by targeting macrophages involved in the pathogenesis of OA. In vitro experiments were performed to evaluate Fpr1 expression during macrophage inflammatory response. In the in vivo studies, anterior cruciate ligament transection (ACLT) surgery was performed and magnetic resonance imaging (MRI) and histological data was assessed to analyze the OA model in both mice and rats. The radioactive probe cFLFLF-PEG- HYNIC-99mTc and SPECT imaging were used to corroborate OA-related inflammation and compare ACLT vs sham knees. In vitro macrophage activation resulted in a remarkable increase in Fpr1 expression. In vivo experiments in mice and rats produced similar results. MRI and histological analysis demonstrated significant joint degeneration in the ACLT knee. The ACLT knee produced a much stronger signal from the probe when compared to the sham knee. It is important to note the ratio of ACLT/sham knee signal intensity decreased with OA progression, indicating greater differences earlier in the progression of OA. The radioactive probe cFLFLF-PEG- HYNIC-99mTc and SPECT imaging is effective for detecting and monitoring inflammation during OA progression by targeting Fpr1 expression in the knee joint.
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DOI：
10.1016/j.clim.2012.12.011
发表时间：
2013-03
期刊：
Clinical immunology (Orlando, Fla.)
影响因子：
--
作者：
Haseeb A;Haqqi TM
通讯作者：
Haqqi TM
影响因子：
7
作者：
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通讯作者：
Bauer, J. S.
影响因子：
7
作者：
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通讯作者：
van den Berg, WB
[99mTc]cFLFLF 用于急性骨髓炎大鼠模型的早期诊断和治疗评估
影响因子：
3.1
作者：
Chen, Jing;Cheng, Hao;Xiong, Wei
通讯作者：
Xiong, Wei
DOI：
10.1016/j.jss.2020.01.028
发表时间：
2020-07
期刊：
The Journal of surgical research
影响因子：
--
作者：
Shannon AH;Chordia MD;Spinosa MD;Su G;Ladd Z;Pan D;Upchurch GR Jr;Sharma AK
通讯作者：
Sharma AK