Whole-transcriptome analysis of rat cavernosum and identification of circRNA-miRNA-mRNA networks to investigate nerve injury erectile dysfunction pathogenesis.
Whole-transcriptome analysis of rat cavernosum and identification of circRNA-miRNA-mRNA networks to investigate nerve injury erectile dysfunction pathogenesis.
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大鼠海绵体全转录组分析及circRNA-miRNA-mRNA网络鉴定研究神经损伤勃起功能障碍发病机制
DOI：
10.1080/21655979.2021.1973863
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发表时间：
2021-12
期刊：
影响因子：
4.9
通讯作者：
Hu Q
中科院分区：
文献类型：
--
作者：
Huang J;Ma J;Wang J;Ma K;Zhou K;Huang W;Zhao F;Lv B;Hu Q
越来越多的证据表明环状RNA（circRNA）在包括勃起功能障碍（艾德）在内的多种疾病中起着重要作用。然而，circRNA在海绵状神经损伤性艾德（CNI-艾德）中的作用尚不清楚。在这里，我们的目的是发现新的circRNA，探讨其在CNI-ED中的潜在作用，并构建一个circRNA的ceRNA网络。将12只雄性Sprague道利大鼠随机分为两组：双侧海绵体神经夹伤组（BCNC）和对照组。手术后四周，使用高通量全转录组测序对大鼠阴茎的海绵状平滑肌组织进行测序。我们分析了两组中circRNAs、miRNAs和mRNAs的表达。选择具有显著不同表达的20种circRNA用于RT-qPCR。使用Cytoscape建立circRNA的CeRNA网络。用R软件包进行GO和KEGG分析。测序显示，BCNC组中有4，587个circRNA，762个miRNAs和21，661个mRNAs失调。通过RT-qPCR进一步验证前20个差异表达的circRNA。ceRNA网络包含10个circRNA、6个miRNA和227个mRNA，包括23个circRNA-miRNA对和227个miRNA-mRNA对。GO和KEGG分析表明，这10种circRNA主要调控能量代谢过程。利用ceRNA网络中的mRNA构建蛋白质-蛋白质相互作用网络，并鉴定出5个枢纽基因。我们的研究揭示了CNI-艾德中circRNA、miRNAs和mRNAs之间的潜在联系，提示circRNA可能通过调节CNI-艾德中细胞的能量代谢而参与艾德的发生。
ABSTRACT There is growing evidence that circular RNAs (circRNAs) play a vital role in many kinds of diseases, including erectile dysfunction (ED). Nevertheless, the role of circRNAs in cavernous nerve-damaging ED (CNI-ED) is unknown. Here, we aimed to discover novel circRNAs, probed their potential role in the CNI-ED, and construct a ceRNA network of circRNAs. Twelve male Sprague Dawley rats were randomly divided into 2 groups by us: bilateral cavernous nerve crush (BCNC) and control groups. Four weeks after surgery, the spongy smooth muscle tissue of the rat penis was sequenced using high-throughput full transcriptome sequencing. We analyzed the expression of circRNAs, miRNAs, and mRNAs in the two groups. Twenty circRNAs with significantly different expressions were selected for RT-qPCR. CeRNA network of circRNAs was established using Cytoscape. GO and KEGG analysis was done by R package. Sequencing showed that 4,587 circRNAs, 762 miRNAs, and 21,661 mRNAs were dysregulated in the BCNC group. The top 20 differentially expressed circRNAs were further verified via RT-qPCR. The ceRNA network contained ten circRNAs, six miRNAs, and 227 mRNAs, including 23 circRNA-miRNA pairs and 227 miRNA-mRNA pairs. GO and KEGG analysis suggested that these ten circRNAs could main regulate energy metabolism processes. A protein‐protein interaction network was constructed with the mRNAs in ceRNA network, and five hub genes were identified. Our study revealed a potential link between circRNAs, miRNAs, and mRNAs in CNI-ED, suggesting that circRNAs may contribute to the occurrence of ED by regulating the cellular energy metabolism in CNI-ED.
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环状 RNA 测序确定 CircASAP1 是肝细胞癌转移的关键调节因子。
影响因子：
13.5
作者：
Hu, Zhi-Qiang;Zhou, Shao-Lai;Zhou, Jian
通讯作者：
Zhou, Jian
影响因子：
4
作者：
Liu, Chun Hua;Jing, Xue Ning;Hou, Chun Hong
通讯作者：
Hou, Chun Hong
影响因子：
3.7
作者：
Shen L;Chen L;Zhang S;Du J;Bai L;Zhang Y;Jiang Y;Li X;Wang J;Zhu L
通讯作者：
Zhu L
影响因子：
4.9
作者：
Sun Z;Zhang A;Hou M;Jiang T
通讯作者：
Jiang T
影响因子：
2.1
作者：
Rehman, J;Christ, G;Fleischmann, J
通讯作者：
Fleischmann, J