Sequencing and characterisation of rearrangements in three S. pastorianus strains reveals the presence of chimeric genes and gives evidence of breakpoint reuse.
Sequencing and characterisation of rearrangements in three S. pastorianus strains reveals the presence of chimeric genes and gives evidence of breakpoint reuse.
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DOI：
10.1371/journal.pone.0092203
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Delneri D
中科院分区：
文献类型：
--
作者：
Hewitt SK;Donaldson IJ;Lovell SC;Delneri D
总的染色体重排有可能在进化上对适应性生物有利。杂交物种的产生通过将两个同源基因组结合在一起来增加重组的机会。我们试图定义的位置基因组重排的三个菌株的酵母巴斯德，一个天然的啤酒酿造酵母杂交酿酒酵母和酵母酵母eubayanus，使用全基因组鸟枪测序。每株S. pastorianus已经失去了其基因组的物种特异性部分，并经历了广泛的重组，产生嵌合染色体。我们预测了30个断点，并通过设计每个断点侧翼的物种特异性引物，然后对PCR产物进行测序，在单核苷酸水平上确认了这些断点。这些重排是两个亚基因组之间同源区域之间重组的结果，而不是重复元件如转座子或tRNA。有趣的是，28/30 S。酿酒酵母- S. Eubayanus重组断点位于基因区域内，产生嵌合基因。此外，我们显示的证据，位于HSP 82和KEM 1，在拟议的独立来源的菌株的重复使用的两个断点。
Gross chromosomal rearrangements have the potential to be evolutionarily advantageous to an adapting organism. The generation of a hybrid species increases opportunity for recombination by bringing together two homologous genomes. We sought to define the location of genomic rearrangements in three strains of Saccharomyces pastorianus, a natural lager-brewing yeast hybrid of Saccharomyces cerevisiae and Saccharomyces eubayanus, using whole genome shotgun sequencing. Each strain of S. pastorianus has lost species-specific portions of its genome and has undergone extensive recombination, producing chimeric chromosomes. We predicted 30 breakpoints that we confirmed at the single nucleotide level by designing species-specific primers that flank each breakpoint, and then sequencing the PCR product. These rearrangements are the result of recombination between areas of homology between the two subgenomes, rather than repetitive elements such as transposons or tRNAs. Interestingly, 28/30 S. cerevisiae- S. eubayanus recombination breakpoints are located within genic regions, generating chimeric genes. Furthermore we show evidence for the reuse of two breakpoints, located in HSP82 and KEM1, in strains of proposed independent origin.
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影响因子：
2.6
作者：
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通讯作者：
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影响因子：
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作者：
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作者：
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通讯作者：
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DOI：
10.1016/j.ijfoodmicro.2007.11.083
发表时间：
2008-02-29
影响因子：
5.4
作者：
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通讯作者：
Querol, Amparo