Sex Differences and Similarities in Hippocampal Cellular Proliferation and the Number of Immature Neurons during Adolescence in Rats
Sex Differences and Similarities in Hippocampal Cellular Proliferation and the Number of Immature Neurons during Adolescence in Rats
复制标题
大鼠青春期海马细胞增殖和未成熟神经元数量的性别差异和相似性
作者：
Alina Siddiqui;R. Romeo
青春期与海马细胞增殖和神经发生的显著减少有关，其生理和行为影响尚不清楚。虽然性别差异存在于成年期的这些增殖过程中，但对性别在这些与增殖有关的变化中所起的作用知之甚少。为了解决这一差距，我们研究了横截面积的齿状回和细胞增殖，Ki-67免疫组织化学测定，在前（30天），中期（45天），青春期后（70天）的雄性和雌性大鼠。我们还调查了未成熟神经元的数量，使用双皮质素（DCX）免疫组织化学在青春期前和青春期后的男性和女性。尽管在青春期的齿状回的大小增加，我们发现显着的海马体增殖在男性和女性，与一个更显着的下降，在男性，表明年龄和性别依赖性的变化在齿状回相关的减少。我们还发现，雄性大鼠齿状回中未成熟神经元数量的下降与肥胖有关，成年大鼠中未成熟神经元数量的性别差异存在女性偏见。鉴于这些显着的变化，在齿状回，这些数据表明，这一时期的发展可能是特别敏感的内部和外部因素已知的调节神经发生，与潜在的性别特异性神经行为的影响。
Adolescence is associated with significant reductions in hippocampal cellular proliferation and neurogenesis, the physiological and behavioral implications of which are unclear. Though sex differences exist in these proliferative processes in adulthood, relatively little is known about the role sex plays in these adolescent-related changes. To address this gap, we examined cross-sectional area of the dentate gyrus and cellular proliferation, as measured by Ki-67 immunohistochemistry, in pre- (30 days), mid- (45 days), and post-adolescent (70 days) male and female rats. We also investigated the number of immature neurons using doublecortin (DCX) immunohistochemistry in pre- and post-adolescent males and females. Despite increases in the size of the dentate gyrus during adolescence, we found significant adolescent-related decreases in hippocampal proliferation in both males and females, with a more dramatic decrease in males, indicating both age- and sex-dependent changes in the dentate gyrus. We also found an adolescent-related decline in the number of immature neurons in the dentate gyrus of male rats and a female-biased sex difference in the number of immature neurons in adults. Given these significant changes in the dentate gyrus, these data suggest that this period in development might be particularly sensitive to internal and external factors known to modulate neurogenesis, with potential sex-specific neurobehavioral ramifications.
DOI：
10.1002/neu.480240705
发表时间：
1993-07
期刊：
Journal of neurobiology
影响因子：
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作者：
Sonia Luquín;Frederick Naftolin;Luis M. Garcia-Segura
通讯作者：
Sonia Luquín;Frederick Naftolin;Luis M. Garcia-Segura