Effect of ischemic preconditioning on interstitial purine metabolite and lactate accumulation during myocardial ischemia.
Effect of ischemic preconditioning on interstitial purine metabolite and lactate accumulation during myocardial ischemia.
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缺血预处理对心肌缺血时间质嘌呤代谢物和乳酸积累的影响。
DOI：
10.1161/01.cir.89.5.2283
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发表时间：
1994
期刊：
影响因子：
37.8
通讯作者：
VanWylen,DG
中科院分区：
文献类型：
--
作者：
VanWylen,DG
本研究旨在探讨缺血预适应对局灶性心肌缺血大鼠心肌间质液(ISF)、嘌呤代谢产物和乳酸的影响。实验分为两组：对照组(n=13)和预适应组(n=10)。在预适应组，诱导局部心肌缺血两次，每次5分钟，然后再灌流10分钟。在这些预适应发作之后，出现了60分钟的持续区域缺血。通过在左室心肌内植入微透析探头采集心脏ISF；透析液水平作为ISF浓度的指标。在预适应组，腺苷、肌苷、次黄嘌呤、总嘌呤和乳酸的浓度在每5分钟的缺血发作期间都有所增加，在再灌流的前5分钟进一步增加。然而，第一次缺血/再灌流时透析液中嘌呤代谢产物的增加大于第二次缺血/再灌流时的增加。此外，与未预适应的动物相比，在长时间的缺血期间，预适应降低了ISF嘌呤代谢产物和乳酸的积累率。这些数据表明，预适应减少了在随后的心肌缺血期间发生的能量失衡，从而减少了腺嘌呤核苷酸净降解率以及细胞产生和释放嘌呤代谢物和乳酸的速度。此外，在预适应期间ISF腺苷的增加与预适应诱导的心脏保护部分是通过腺苷在细胞外A1腺苷受体上的作用而介导的观点是一致的。
The purpose of this study was to determine the effect of ischemic preconditioning on the changes in interstitial fluid (ISF) purine metabolites and lactate during prolonged regional myocardial ischemia. The study consisted of two groups of anesthetized dogs: a control group (n = 13) that was exposed to 60 minutes of regional myocardial ischemia and a preconditioned group (n = 10). In the preconditioned group, regional myocardial ischemia was induced for two 5-minute episodes, each followed by 10 minutes of reperfusion. These preconditioning episodes were followed by 60 minutes of sustained regional ischemia. Cardiac ISF was sampled by microdialysis probes implanted in the left ventricular myocardium; dialysate levels served as indices of ISF concentrations. In the preconditioned group, dialysate concentrations of adenosine, inosine, hypoxanthine, total purines, and lactate increased during each of the 5-minute ischemia episodes, with further increases occurring during the first 5 minutes of reperfusion. However, the increases in dialysate purine metabolites during the first period of ischemia/reperfusion were greater than those that occurred during the second ischemia/reperfusion. In addition, preconditioning reduced the rate of ISF purine metabolite and lactate accumulation during the prolonged ischemia when compared with nonpreconditioned animals. These data suggest that preconditioning reduces the energy imbalance that occurs during subsequent myocardial ischemia and thereby diminishes the rate of net adenine nucleotide degradation and cellular production and release of purine metabolites and lactate. In addition, the increase in ISF adenosine seen during the preconditioning episode is consistent with the notion that preconditioning-induced cardioprotection is mediated in part by the action of adenosine at extracellular A1 adenosine receptors.
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