Protection of IFNAR (-/-) mice against bluetongue virus serotype 8, by heterologous (DNA/rMVA) and homologous (rMVA/rMVA) vaccination, expressing outer-capsid protein VP2.
Protection of IFNAR (-/-) mice against bluetongue virus serotype 8, by heterologous (DNA/rMVA) and homologous (rMVA/rMVA) vaccination, expressing outer-capsid protein VP2.
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DOI：
10.1371/journal.pone.0060574
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Castillo-Olivares J
中科院分区：
文献类型：
--
作者：
Jabbar TK;Calvo-Pinilla E;Mateos F;Gubbins S;Bin-Tarif A;Bachanek-Bankowska K;Alpar O;Ortego J;Takamatsu HH;Mertens PP;Castillo-Olivares J
在小鼠模型中测试表达血清型8蓝舌病病毒（BTV-8）衣壳蛋白的重组疫苗的保护效力。重组疫苗包含编码BTV VP 2、VP 5或VP 7蛋白的质粒DNA或修饰的安卡拉牛痘病毒。这些构建体单独施用或使用同源初免加强接种方案（rMVA/rMVA）或异源接种方案（DNA/rMVA）组合施用。DNA/rMVA或rMVA/rMVA初免-加强以三周间隔施用，并且接受VP 2的所有动物产生中和抗体。随后用致死剂量的BTV-8攻击接种疫苗和未接种疫苗的对照小鼠。单独用VP 7接种的小鼠不受保护。然而，用DNA/rMVA或表达VP 2、VP 5和VP 7的rMVA/rMVA或单独的VP 2接种的小鼠都受到保护。
The protective efficacy of recombinant vaccines expressing serotype 8 bluetongue virus (BTV-8) capsid proteins was tested in a mouse model. The recombinant vaccines comprised plasmid DNA or Modified Vaccinia Ankara viruses encoding BTV VP2, VP5 or VP7 proteins. These constructs were administered alone or in combination using either a homologous prime boost vaccination regime (rMVA/rMVA) or a heterologous vaccination regime (DNA/rMVA). The DNA/rMVA or rMVA/rMVA prime-boost were administered at a three week interval and all of the animals that received VP2 generated neutralising antibodies. The vaccinated and non-vaccinated-control mice were subsequently challenged with a lethal dose of BTV-8. Mice vaccinated with VP7 alone were not protected. However, mice vaccinated with DNA/rMVA or rMVA/rMVA expressing VP2, VP5 and VP7 or VP2 alone were all protected.
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影响因子：
3.7
作者：
Calvo-Pinilla E;Navasa N;Anguita J;Ortego J
通讯作者：
Ortego J
影响因子：
5.5
作者：
Franceschi, Valentina;Capocefalo, Antonio;Donofrio, Gaetano
通讯作者：
Donofrio, Gaetano
影响因子：
5.5
作者：
Fournillier, A.;Gerossier, E.;Inchauspe, G.
通讯作者：
Inchauspe, G.
影响因子：
64.8
作者：
Grimes, JM;Burroughs, JN;Stuart, DI
通讯作者：
Stuart, DI
影响因子：
6.9
作者：
Golde, WT;Gollobin, P;Rodriguez, LL
通讯作者：
Rodriguez, LL