Synaptotagmin-1 and synaptotagmin-7 trigger synchronous and asynchronous phases of neurotransmitter release.
Synaptotagmin-1 and synaptotagmin-7 trigger synchronous and asynchronous phases of neurotransmitter release.
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DOI：
10.1016/j.neuron.2013.10.026
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发表时间：
2013-11-20
期刊：
影响因子：
16.2
通讯作者：
Südhof TC
中科院分区：
文献类型：
--
作者：
Bacaj T;Wu D;Yang X;Morishita W;Zhou P;Xu W;Malenka RC;Südhof TC
In forebrain neurons, knockout of synaptotagmin-1 blocks fast Ca2+-triggered synchronous neurotransmitter release, but enables manifestation of slow Ca2+-triggered asynchronous release. Here, we show using single-cell PCR that individual hippocampal neurons abundantly co-express two Ca2+-binding synaptotagmin isoforms, synaptotagmin-1 and synaptotagmin-7. In synaptotagmin-1 deficient synapses of excitatory and inhibitory neurons, loss-of-function of synaptotagmin-7 suppressed asynchronous release. This phenotype was rescued by wild-type but not mutant synaptotagmin-7 lacking functional Ca2+-binding sites. Even in synaptotagmin-1 containing neurons, synaptotagmin-7 ablation partly impaired asynchronous release induced by extended high-frequency stimulus trains. Synaptotagmins bind Ca2+ via two C2-domains, the C2A- and C2B-domains. Surprisingly, synaptotagmin-7 function selectively required its C2A-domain Ca2+-binding sites, whereas synaptotagmin-1 function required its C2B-domain Ca2+-binding sites. Our data show that nearly all Ca2+-triggered release at a synapse is due to synaptotagmins, with synaptotagmin-7 mediating a slower form of Ca2+-triggered release that is normally occluded by faster synaptotagmin-1-induced release, but becomes manifest upon synaptotagmin-1 deletion.
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影响因子：
64.5
作者：
Cao P;Maximov A;Südhof TC
通讯作者：
Südhof TC
影响因子：
64.5
作者：
GEPPERT, M;GODA, Y;SUDHOF, TC
通讯作者：
SUDHOF, TC
DOI：
10.1073/pnas.0712372105
发表时间：
2008-03-11
影响因子：
11.1
作者：
Maximov, Anton;Lao, Ye;Suedhof, Thomas C.
通讯作者：
Suedhof, Thomas C.
影响因子：
56.9
作者：
BROSE, N;PETRENKO, AG;JAHN, R
通讯作者：
JAHN, R
DOI：
10.1523/jneurosci.3214-12.2013
发表时间：
2013-01-02
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Lee J;Guan Z;Akbergenova Y;Littleton JT
通讯作者：
Littleton JT