Engineering Breast Cancer Microenvironments and 3D Bioprinting.
Engineering Breast Cancer Microenvironments and 3D Bioprinting.
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DOI：
10.3389/fbioe.2018.00066
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发表时间：
2018
影响因子：
5.7
通讯作者：
Jung JP
中科院分区：
文献类型：
--
作者：
Belgodere JA;King CT;Bursavich JB;Burow ME;Martin EC;Jung JP
细胞外基质（ECM）是指导肿瘤发生和转移的关键因子。虽然二维（2D）培养模型已被广泛用于了解乳腺癌微环境在过去的几十年里，2D模型仍然表现出有限的成功。压倒性的证据支持，需要三维（3D）生理相关的培养模型来更好地了解癌症进展并开发更有效的治疗方法。此类平台应包括癌症特异性结构、相关理化信号、基质-癌细胞相互作用、免疫组分、血管组分和患者肿瘤中发现的细胞-ECM相互作用。这篇综述简要总结了如何定义癌症微环境（基质成分，细胞-ECM相互作用和分子调节剂），以及可以利用哪些新兴技术（可灌注支架，肿瘤硬度，肿瘤内的支持细胞和复杂的图案）来更好地模拟天然样乳腺癌微环境。此外，这篇综述强调了原发性肿瘤ECM和转移性病变组织部位之间的生物物理特性不同，重点是癌症干细胞的基质调节，为研究可能改变患者预后的未充分探索的ECM蛋白提供了理论基础。为了设计乳腺癌微环境，我们将技术分为两组：（1）调节乳腺癌细胞-ECM相互作用的生化因素和（2）3D生物打印方法及其在乳腺癌微环境建模中的应用。生化因子包括基质相关蛋白、可溶性因子、ECM和合成生物材料。对于3D生物打印的应用，我们讨论了2D图案到3D支架的过渡，采用各种生物打印技术来实现生物物理线索来模拟乳腺癌微环境。
The extracellular matrix (ECM) is a critical cue to direct tumorigenesis and metastasis. Although two-dimensional (2D) culture models have been widely employed to understand breast cancer microenvironments over the past several decades, the 2D models still exhibit limited success. Overwhelming evidence supports that three dimensional (3D), physiologically relevant culture models are required to better understand cancer progression and develop more effective treatment. Such platforms should include cancer-specific architectures, relevant physicochemical signals, stromal–cancer cell interactions, immune components, vascular components, and cell-ECM interactions found in patient tumors. This review briefly summarizes how cancer microenvironments (stromal component, cell-ECM interactions, and molecular modulators) are defined and what emerging technologies (perfusable scaffold, tumor stiffness, supporting cells within tumors and complex patterning) can be utilized to better mimic native-like breast cancer microenvironments. Furthermore, this review emphasizes biophysical properties that differ between primary tumor ECM and tissue sites of metastatic lesions with a focus on matrix modulation of cancer stem cells, providing a rationale for investigation of underexplored ECM proteins that could alter patient prognosis. To engineer breast cancer microenvironments, we categorized technologies into two groups: (1) biochemical factors modulating breast cancer cell-ECM interactions and (2) 3D bioprinting methods and its applications to model breast cancer microenvironments. Biochemical factors include matrix-associated proteins, soluble factors, ECMs, and synthetic biomaterials. For the application of 3D bioprinting, we discuss the transition of 2D patterning to 3D scaffolding with various bioprinting technologies to implement biophysical cues to model breast cancer microenvironments.
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DOI：
10.1016/j.mattod.2015.05.002
发表时间：
2015-12
期刊：
Materials today (Kidlington, England)
影响因子：
--
作者：
Asghar W;El Assal R;Shafiee H;Pitteri S;Paulmurugan R;Demirci U
通讯作者：
Demirci U
DOI：
10.1186/s13058-016-0740-2
发表时间：
2016-08-11
期刊：
Breast cancer research : BCR
影响因子：
--
作者：
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通讯作者：
Marini FC
影响因子：
16.6
作者：
Akthar S;Patel DF;Beale RC;Peiró T;Xu X;Gaggar A;Jackson PL;Blalock JE;Lloyd CM;Snelgrove RJ
通讯作者：
Snelgrove RJ
影响因子：
3.7
作者：
Barcus CE;Holt EC;Keely PJ;Eliceiri KW;Schuler LA
通讯作者：
Schuler LA
影响因子：
4.7
作者：
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通讯作者：
Cui, Xiaofeng