Ultrastructural morphology and relaxin immunolocalization in giant trophoblast cells of the golden hamster placenta.
Ultrastructural morphology and relaxin immunolocalization in giant trophoblast cells of the golden hamster placenta.
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金仓鼠胎盘巨型滋养层细胞的超微结构形态和松弛素免疫定位。
作者：
T. Johns;R. Renegar
松弛素免疫反应性先前在仓鼠胎盘内的三种细胞类型中得到证实；胎儿原发性和继发性巨滋养细胞（GTCs）和母体子宫内膜粒细胞。本研究的目的是研究gtc的超微结构，并确定细胞内松弛素的储存位点。初代gtc在妊娠第8天首次出现，其特征是有大量的多核糖体和大的异质性细胞质包涵体，表明具有吞噬活性。妊娠第10、14和15天的初级和次级gtc含有大量的多核糖体、管状嵴线粒体、广泛的高尔基复合体和丰富的粗内质网，这些都是细胞积极参与蛋白质合成的特征。未见膜结合性分泌颗粒。利用亲和素-生物素-过氧化物酶法在初级和次级gtc的高尔基复合体中免疫定位松弛素。对仓鼠胎盘匀浆进行差速离心和亚细胞部分放射免疫分析（RIA）后，在微粒体后部分检测到大部分松弛素免疫活性；然而，类似处理的猪黄体的松弛素免疫活性主要存在于线粒体/颗粒部分。这些数据表明，仓鼠胎盘松弛素不是储存在膜结合的分泌颗粒中，而是包含在广泛的GTC高尔基复合体中。
Relaxin immunoreactivity was previously demonstrated in three cell types within the hamster placenta; fetal primary and secondary giant trophoblast cells (GTCs) and maternal endometrial granulocytes. The objectives of the present research were to examine the ultrastructure of the GTCs and identify the intracellular relaxin storage site. Primary GTCs, first present on day 8 of gestation, were characterized by numerous polyribosomes and large heterogeneous cytoplasmic inclusions suggesting phagocytic activity. Primary and secondary GTCs from days 10, 14, and 15 of gestation contained numerous polyribosomes, mitochondria with tubular cristae, and extensive Golgi complex, and abundant rough endoplasmic reticulum, all characteristics of a cell actively involved in protein synthesis. Membrane-bound secretory granules were not present. Relaxin was immunolocalized within the Golgi complex of primary and secondary GTCs using the avidin-biotin-peroxidase method. Following differential centrifugation of hamster placental homogenates and radioimmunoassay (RIA) of subcellular fractions, the majority of relaxin immunoactivity was detected in the postmicrosomal fraction; however, the majority of relaxin immunoactivity from similarly treated pig corpora lutea was present in the mitochondrial/granule fraction. These data indicate that hamster placental relaxin is not stored in membrane-bound secretory granules but is contained within the extensive Golgi complex of the GTC.
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松弛素在怀孕大鼠中的生理作用。
影响因子：
4.8
作者：
Downing,SJ;Sherwood,OD
通讯作者：
Sherwood,OD
妊娠后半期金仓鼠（Mesocricetus auratus）松弛素的免疫细胞化学定位。
影响因子：
3.6
作者：
Renegar,RH;Cobb,AD;Leavitt,WW
通讯作者：
Leavitt,WW
妊娠大鼠黄体松弛素免疫反应性的超微结构定位。
影响因子：
4.8
作者：
Anderson,MB;Sherwood,OD
通讯作者：
Sherwood,OD
松弛素在怀孕大鼠中的生理作用。
影响因子：
4.8
作者：
Downing,SJ;Sherwood,OD
通讯作者：
Sherwood,OD