Tumor-specific fluorescence antibody imaging enables accurate staging laparoscopy in an orthotopic model of pancreatic cancer.
Tumor-specific fluorescence antibody imaging enables accurate staging laparoscopy in an orthotopic model of pancreatic cancer.
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DOI：
10.5754/hge11836
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发表时间：
2012-09
影响因子：
--
通讯作者：
Bouvet M
中科院分区：
文献类型：
--
作者：
Tran Cao HS;Kaushal S;Metildi CA;Menen RS;Lee C;Snyder CS;Messer K;Pu M;Luiken GA;Talamini MA;Hoffman RM;Bouvet M
腹腔镜检查在胰腺癌分期中很重要，但假阴性仍然存在问题。使肿瘤发出荧光有可能提高腹腔镜分期的准确性。用BxPC-3人胰腺癌细胞建立裸鼠胰腺癌原位和转移模型。在腹腔镜检查前24小时通过尾静脉注射Alexa 488-抗CEA缀合物。在明视野腹腔镜（BL）和荧光腹腔镜（FL）模式下检查小鼠。测量的结果包括原位模型识别原发性肿瘤的时间和癌病模型在2分钟内识别的转移灶数量。与BL相比，FL能够更快速准确地识别和定位原发肿瘤和转移瘤。使用BL比FL花费的时间在统计学上显著更长。与BL相比，FL下检测到和定位的转移性病变更多，并且具有更高的准确性，与对照相比，灵敏度分别为96%和40%。FL对<1 mm的转移灶有足够的敏感性。使用荧光腹腔镜检查与荧光团标记的抗CEA抗体标记的肿瘤，允许快速检测和准确定位的原发性和转移性胰腺癌在原位模型。本报告的结果证明了荧光腹腔镜的未来临床潜力。
Laparoscopy is important in staging pancreatic cancer, but false negatives remain problematic. Making tumors fluorescent has the potential to improve the accuracy of staging laparoscopy. Orthotopic and carcinomatosis models of pancreatic cancer were established with BxPC-3 human pancreatic cancer cells in nude mice. Alexa488-anti-CEA conjugates were injected via tail vein 24 hours prior to laparoscopy. Mice were examined under bright field laparoscopic (BL) and fluorescence laparoscopic (FL) modes. Outcomes measured included time to identification of primary tumor for the orthotopic model and number of metastases identified within 2 minutes for the carcinomatosis model. FL enabled more rapid and accurate identification and localization of primary tumors and metastases than BL. Using BL took statistically significantly longer time than FL. More metastatic lesions were detected and localized under FL compared to BL and with greater accuracy, with sensitivities of 96% vs. 40%, respectively, when compared to control. FL was sensitive enough to detect metastatic lesions <1mm. The use of fluorescence laparoscopy with tumors labeled with fluorophore-conjugated anti-CEA antibody permits rapid detection and accurate localization of primary and metastatic pancreatic cancer in an orthotopic model. The results of the present report demonstrate the future clinical potential of fluorescence laparoscopy.
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影响因子：
4.9
作者：
Hu CM;Kaushal S;Tran Cao HS;Aryal S;Sartor M;Esener S;Bouvet M;Zhang L
通讯作者：
Zhang L
影响因子：
--
作者：
Jimenez, RE;Warshaw, AL;Fernandez-del Castillo, C
通讯作者：
Fernandez-del Castillo, C
影响因子：
9
作者：
Gahlen, J;Prosst, RL;Herfarth, C
通讯作者：
Herfarth, C
影响因子：
2.5
作者：
YAMAGUCHI, K;ENJOJI, M;TSUNEYOSHI, M
通讯作者：
TSUNEYOSHI, M
影响因子：
9
作者：
Conlon, KC;Dougherty, E;Brennan, MF
通讯作者：
Brennan, MF