Contribution of left ventricular residual stress by myocytes and collagen: existence of inter-constituent mechanical interaction.
Contribution of left ventricular residual stress by myocytes and collagen: existence of inter-constituent mechanical interaction.
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DOI：
10.1007/s10237-018-1007-x
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发表时间：
2018-08
影响因子：
3.5
通讯作者：
Roccabianca S
中科院分区：
文献类型：
--
作者：
Grobbel MR;Shavik SM;Darios E;Watts SW;Lee LC;Roccabianca S
我们量化的贡献，心肌细胞，胶原纤维和它们之间的相互作用，在左心室（LV）中发现的残余应力场，使用实验和理论方法。从正常大鼠（雄性和雌性）左心室提取的环组织样品分别用胶原酶和脱细胞处理以分离肌细胞和胶原纤维。然后对这些样品以及作为对照的含有两种成分的完整组织样品进行开角试验。我们的研究结果表明，胶原纤维是在左心室中发现的残余应力场的主要贡献者。具体而言，仅胶原样品（106.45°±23.02°）和仅肌细胞样品（21.00°±4.37°）中测量的张开角分别显著高于和低于对照样品（57.88°±12.29°）。然后使用约束混合物（CM）建模框架来推断这些实验结果。通过对仅含胶原蛋白和仅含肌细胞的样本进行测量，我们表明该框架无法重现完整组织中发现的张开角。鉴于CM框架假设每个组分仅通过其存在而对整体力学做出贡献，该结果表明在LV中存在一些不可忽视的肌细胞-胶原机械相互作用。然后，我们提出了一个扩展的CM配方，考虑到成分间的机械相互作用，其中成分变形时，它们被物理组合成一个混合物。我们表明，完整的组织张开角可以恢复在这个框架。
We quantify the contribution of myocytes, collagen fibers and their interactions to the residual stress field found in the left ventricle (LV) using both experimental and theoretical methods. Ring tissue samples extracted from normal rat, male and female, LV were treated with collagenase and decellularization to isolate myocytes and collagen fibers, respectively. Opening angle tests were then performed on these samples as well as intact tissue samples containing both constituents that served as control. Our results show that the collagen fibers are the main contributor to the residual stress fields found in the LV. Specifically, opening angle measured in collagen-only samples (106.45°±23.02°) and myocytes-only samples (21.00°±4.37°) was significantly higher and lower than that of the control (57.88°±12.29°), respectively. A constrained mixture (CM) modeling framework was then used to infer these experimental results. We show that the framework cannot reproduce the opening angle found in the intact tissue with measurements made on the collagen-only and myocytes-only samples. Given that the CM framework assumes that each constituent contributes to the overall mechanics simply by their mere presence, this result suggests the existence of some myocyte–collagen mechanical interaction that cannot be ignored in the LV. We then propose an extended CM formulation that takes into account of the inter-constituent mechanical interaction in which constituents are deformed additionally when they are physically combined into a mixture. We show that the intact tissue opening angle can be recovered in this framework.
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影响因子：
9.5
作者：
MacKenna, DA;Omens, JH;Covell, JW
通讯作者：
Covell, JW
影响因子：
3.5
作者：
Omens, J. H.;McCulloch, A. D.;Criscione, J. C.
通讯作者：
Criscione, J. C.
影响因子：
20.1
作者：
OMENS, JH;FUNG, YC
通讯作者：
FUNG, YC
影响因子：
10.8
作者：
MEDUGORAC, I;JACOB, R
通讯作者：
JACOB, R
DOI：
10.1073/pnas.1203007109
发表时间：
2012-06-19
影响因子：
11.1
作者：
McCain, Megan L.;Lee, Hyungsuk;Parker, Kevin Kit
通讯作者：
Parker, Kevin Kit