The Protective Effect of Metformin Use on Early Nd:YAG Laser Capsulotomy.
The Protective Effect of Metformin Use on Early Nd:YAG Laser Capsulotomy.
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DOI：
10.1167/iovs.62.10.24
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发表时间：
2021-08-02
影响因子：
4.4
通讯作者：
Petrash JM
中科院分区：
文献类型：
--
作者：
Patnaik JL;Christopher KL;Pedler MG;Shieh B;Petrash CC;Wagner BD;Mandava N;Lynch AM;Palestine AG;Petrash JM
目的：探讨二甲双胍对后囊混浊(PCO)早期激光治疗的影响，并探讨其可能的分子机制。我们进行了：1)在我所进行的白内障超声乳化术患者眼的回顾性队列研究；2)在后囊混浊动物模型的背景下，对二甲双胍对晶状体上皮细胞行为的影响进行了实验室研究。使用总体平均的COX比例风险模型来估计发生ND：YAG的时间的风险。在实验室研究中，在组织培养和囊外晶状体摘除后的小鼠模型中，测量了与PCO发病机制有关的上皮向间充质转化(EMT)标志物的表达。9798只眼中，激光切开率为13.1%。单因素分析显示，二甲双胍的使用和糖尿病是影响激光囊膜切开术的保护因素。然而，在以非糖尿病患者为参照组的多因素分析中，只有糖尿病患者使用二甲双胍对其有显著的保护作用(危险比：0.68，95%CI：0.54~0.85，P=0.0008)，而糖尿病患者的眼部无明显差异(P=0.5026)。用二甲双胍处理晶状体上皮细胞可降低转化生长因子-SMA-2诱导的晶状体上皮细胞⍺-SMA和PERK的表达水平。类似地，在小鼠晶状体囊外摘除模型中，二甲双胍处理减少晶状体上皮细胞⍺-SMA的表达。二甲双胍对早期晶体上皮细胞的保护作用可能与其下调残留晶状体上皮细胞的EMT有关，而残余晶状体上皮细胞倾向于肌成纤维细胞的发育和后囊混浊。
To determine the effect of metformin on early Nd:YAG laser treatment for posterior capsule opacification (PCO) and to explore a molecular mechanism to explain a possible protective effect of metformin against PCO. We conducted: 1) a retrospective cohort study of patient eyes undergoing phacoemulsification at our institution; and 2) laboratory investigation of the effect of metformin on the behavior of lens epithelial cells in the context of an animal model for PCO. Population-averaged Cox proportional hazards modeling was used to estimate risk for time to Nd:YAG. For laboratory studies, expression of markers for epithelial-to-mesenchymal transition (EMT) implicated in PCO pathogenesis was measured in tissue culture and following extracapsular lens extraction in a mouse model. The rate of Nd:YAG laser capsulotomy was 13.1% among the 9798 eyes. Both metformin use and diabetes were protective factors for Nd:YAG laser capsulotomy in univariate analysis. However, in multivariable analysis with nondiabetics as the reference group, only metformin use among diabetics was significantly protective of Nd:YAG (hazard ratio: 0.68, 95% CI: 0.54–0.85, P = 0.0008), while eyes of patients with diabetes without metformin use did not significantly differ (P = 0.5026). Treatment of lens epithelial cells with metformin reduced the level of the EMT markers ⍺-SMA and pERK induced by TGF-β2. Similarly, metformin treatment reduced ⍺-SMA expression in lens epithelial cells following extracapsular lens extraction in a mouse model. The protective effect of metformin against early Nd:YAG may relate to its ability to downregulate EMT in residual lens epithelial cells that otherwise trend toward myofibroblast development and PCO.
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影响因子：
2.8
作者：
Ando, H;Ando, N;Oshika, T
通讯作者：
Oshika, T
影响因子：
2.8
作者：
Leydolt, Christina;Schartmueller, Daniel;Menapace, Rupert
通讯作者：
Menapace, Rupert
DOI：
10.1034/j.1600-0420.2003.00091.x
发表时间：
2003-08-01
期刊：
ACTA OPHTHALMOLOGICA SCANDINAVICA
影响因子：
--
作者：
Stordahl, PB;Drolsum, L
通讯作者：
Drolsum, L
影响因子：
2.8
作者：
Georgopoulos, M;Findl, O;Rainer, G
通讯作者：
Rainer, G
影响因子：
4.8
作者：
Mishra, Rangnath;Cool, Barbara L.;Simonson, Michael S.
通讯作者：
Simonson, Michael S.