A Dynamic Functional Stem Cell Microtissue Culture System Based on Magnetic Nanoparticles Labeled Endothelial Cells
A Dynamic Functional Stem Cell Microtissue Culture System Based on Magnetic Nanoparticles Labeled Endothelial Cells
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基于磁性纳米颗粒标记内皮细胞的动态功能干细胞微组织培养系统
DOI：
10.1016/j.apmt.2020.100691
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发表时间：
2020-09
影响因子：
8.3
通讯作者：
Jiang Xinquan
中科院分区：
文献类型：
--
作者：
Yang Guangzheng;Lu Yuezhi;Liu Chang;Zhou Mingliang;Yin Shi;Zhang Wenjie;Jiang Xinquan
活体微型机器人的三维（3D）多细胞结构通过心肌细胞的收缩被赋予了运动能力，可能是未来的强大工具。受此启发，我们开发了一种基于内皮细胞的动态功能微组织培养系统。与表现出节律性收缩的心肌细胞或肌肉细胞相反，我们发现含有人脐静脉内皮细胞（HUVEC）的细胞片可以自发地缓慢收缩并卷曲成碗状微组织。在微组织中，HUVEC 形成管状结构，这可能会增强血管生成潜力，这一功能有利于维持微组织的增殖。通过磁控技术选择牙髓干细胞（DPSC）纳入动态功能微组织中。随着培养时间的延长，含有半内皮细胞的HUVECs/DPSCs复合微组织仍然表现出收缩和卷曲效应。更重要的是，收缩过程显着增强了复合微组织中成骨相关基因和蛋白质的表达。初步研究结果表明，这种动态功能微组织未来在许多领域，特别是骨再生方面具有广阔的应用前景。
Living microrobots, in which three-dimensional (3D) multicellular structures are endowed with movement ability through the contraction of cardiac myocytes, may be powerful tools in the future. Inspired by this, we developed a dynamic functional microtissue culture system based on endothelial cells. In contrast to cardiac myocytes or muscle cells, which exhibit rhythmic contraction, we found that cell sheets containing human umbilical vein endothelial cells (HUVECs) could spontaneously and slowly contract and curl into a bowl-like microtissue. In the microtissue, the HUVECs formed tubular structures, which might enhance the angiogenic potential, a function that is beneficial for maintaining the proliferation of microtissue. Dental pulp stem cells (DPSCs) were selected to be incorporated in the dynamic functional microtissue through magnetic controlled technology. With prolonged culture time, the HUVECs/DPSCs composite microtissue containing half endothelial cells still exhibited contraction and curling effects. More importantly, the contraction process significantly enhanced the expression of osteogenesis-related genes and proteins in the composite microtissue. The preliminary research results indicated that this dynamic functional microtissue may hold great promise for future use in many fields, especially in bone regeneration.
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影响因子：
19
作者：
Goodfellow F;Simchick GA;Mortensen LJ;Stice SL;Zhao Q
通讯作者：
Zhao Q
DOI：
10.1039/c4ib00219a
发表时间：
2015-02
期刊：
Integrative biology : quantitative biosciences from nano to macro
影响因子：
--
作者：
V. Du;D. Fayol;M. Reffay;N. Luciani;J. Bacri;C. Gay;C. Wilhelm
通讯作者：
V. Du;D. Fayol;M. Reffay;N. Luciani;J. Bacri;C. Gay;C. Wilhelm
DOI：
10.1113/jphysiol.1996.sp021275
发表时间：
1998-06
期刊：
The Journal of Physiology
影响因子：
--
作者：
R. Greenwood;O. Scott;H. P.E.M.;Habets;-D.;Franco;J. A.;Sargeantt;J. Pereira;A. Moorman
通讯作者：
R. Greenwood;O. Scott;H. P.E.M.;Habets;-D.;Franco;J. A.;Sargeantt;J. Pereira;A. Moorman
影响因子：
4.9
作者：
Whatley, Benjamin R.;Li, Xiaowei;Wen, Xuejun
通讯作者：
Wen, Xuejun
利用细胞片技术和超小型超顺磁性氧化铁标记制造组织工程仿生尿道，用于全层尿道重建
影响因子：
12.4
作者：
Zhou S;Yang R;Zou Q;Zhang K;Yin T;Zhao W;Shapter JG;Gao G;Fu Q
通讯作者：
Fu Q