The DEAD box protein Mrh4 functions in the assembly of the mitochondrial large ribosomal subunit.
The DEAD box protein Mrh4 functions in the assembly of the mitochondrial large ribosomal subunit.
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DOI：
10.1016/j.cmet.2013.10.007
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发表时间：
2013-11-05
期刊：
影响因子：
29
通讯作者：
Barrientos A
中科院分区：
文献类型：
--
作者：
De Silva D;Fontanesi F;Barrientos A
细胞中的蛋白质普遍由核糖体合成。线粒体含有自己的核糖体，专门合成氧化磷酸化所需的少数蛋白质。线粒体生物发生的途径和相关因素的特点很差。一个恰当的例子是DEAD-Box蛋白，众所周知，它作为RNA解旋酶或RNA伴侣参与细菌和细胞质真核生物核糖体的生物合成，而其线粒体对应物仍然完全未知。在这里，我们已经确定酿酒酵母线粒体DEAD-Box蛋白Mrh 4对于大线粒体亚基生物发生至关重要。Mrh 4与21 S rRNA、线粒体核糖体和完全组装的线粒体核糖体相互作用。在不存在Mrh 4的情况下，21 S rRNA成熟并形成大的通路上组装中间缺失蛋白Mrpl 16和Mrpl 39的一部分。我们的结论是，Mrh 4起着至关重要的作用，在后期阶段的线粒体组装，通过促进重塑的21 S rRNA-蛋白质相互作用。
Proteins in a cell are universally synthesized by ribosomes. Mitochondria contain their own ribosomes, which specialize on the synthesis of a handful of proteins required for oxidative phosphorylation. The pathway of mitoribosomal biogenesis and factors involved are poorly characterized. A case in point are the DEAD-Box proteins, widely known to participate in the biogenesis of bacterial and cytoplasmic eukaryotic ribosomes as either RNA helicases or RNA chaperones, whose mitochondrial counterparts remain completely unknown. Here, we have identified the Saccharomyces cerevisiae mitochondrial DEAD-Box protein Mrh4 as essential for large mitoribosome subunit biogenesis. Mrh4 interacts with the 21S rRNA, mitoribosome subassemblies and fully assembled mitoribosomes. In the absence of Mrh4, the 21S rRNA is matured and forms part of a large on-pathway assembly intermediate missing proteins Mrpl16 and Mrpl39. We conclude that Mrh4 plays an essential role during the late stages of mitoribosome assembly by promoting remodeling of the 21S rRNA-protein interactions.
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影响因子：
29
作者：
Camara, Yolanda;Asin-Cayuela, Jorge;Larsson, Nils-Goran
通讯作者：
Larsson, Nils-Goran
影响因子：
14.9
作者：
Sharpe Elles LM;Sykes MT;Williamson JR;Uhlenbeck OC
通讯作者：
Uhlenbeck OC
影响因子：
11.4
作者：
Barrientos, A;Zambrano, A;Tzagoloff, A
通讯作者：
Tzagoloff, A
影响因子：
64.8
作者：
Gavin, AC;Bösche, M;Superti-Furga, G
通讯作者：
Superti-Furga, G
DOI：
10.1073/pnas.0407896101
发表时间：
2005-01-04
影响因子：
11.1
作者：
Huang, HR;Rowe, CE;Perlman, PS
通讯作者：
Perlman, PS