Antiendothelial cell antibodies can be cytotoxic to endothelial cells without cytokine pre‐stimulation and correlate with ELISA antibody measurement in Kawasaki disease
Antiendothelial cell antibodies can be cytotoxic to endothelial cells without cytokine pre‐stimulation and correlate with ELISA antibody measurement in Kawasaki disease
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抗内皮细胞抗体可以在没有细胞因子预刺激的情况下对内皮细胞产生细胞毒性，并与川崎病的 ELISA 抗体测量相关
DOI：
10.1111/j.1365-2249.1994.tb06136.x
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发表时间：
1994
影响因子：
4.6
通讯作者：
M. Dillon
中科院分区：
文献类型：
--
作者：
K. Kaneko;C. Savage;B. Pottinger;V. Shah;J. Pearson;M. Dillon
为了确认抗内皮细胞抗体（AECA）的存在并研究其测量在川崎（KD）中的临床价值，使用基于细胞的ELISA评估了KD患者血清与人脐静脉内皮细胞（HUVEC）的结合，并使用标记的HUVEC评估了补体介导的细胞毒性。在ELISA试验中，IgM AECA在16/22份血清中检出，而IgG AECA仅在1/19份血清中检出。有一个显着差异IgM AECA滴度比较患者与对照组。16份血清中有8份显示出对HUVEC的细胞毒性，并且当HUVEC用肿瘤坏死因子（TNF）预处理时，这种细胞毒性显著增强。ELISA法测定的IgM AECA滴度与其细胞毒活性呈正相关。结果表明，KD患者血清中AECA类IgM抗体可用ELISA法检测，其中部分抗体可通过ELISA法检测。在本研究中，50%可介导针对内皮细胞的补体依赖性细胞毒性。
To confirm the presence of antiendothelial cell antibodies (AECA) and study the clinical value of their measurement in Kawasaki disease (KD), sera from patients with KD were assessed for binding to human umbilieal vein endothelial cells (HUVEC) using a cellular based ELISA, and for complement‐mediated cytotoxiety using in‐labelled HUVEC. In the ELISA assay, IgM AECA were detected in 16/22 sera, whereas IgG AECA were only present in one out of 19 sera. There was a significant difference in IgM AECA titres when comparing patients with controls. Eight out of 16 sera showed cytotoxicity against HUVEC, and this was significantly enhanced when HUVEC were pretreated with tumour neerosis factor (TNF). IgM AECA titres measured by ELISA were positively correlated with their cytotoxieity. The findings suggest that IgM class AECA in sera from patients with KD are detectable by ELISA method, and that some of these antibodies. 50% in this study, can mediate complement‐dependent cytotoxicity against endothelial cells.
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影响因子：
--
作者：
D. Leung
通讯作者：
D. Leung
影响因子：
4.4
作者：
T. Collins;A. Krensky;C. Clayberger;W. Fiers;M. Gimbrone;S. Burakoff;J. Pober
通讯作者：
T. Collins;A. Krensky;C. Clayberger;W. Fiers;M. Gimbrone;S. Burakoff;J. Pober
粒细胞介导的单纯疱疹病毒感染的人内皮损伤。
DOI：
--
发表时间：
1989
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Visser,MR;Jacob,HS;Goodman,JL;McCarthy,JB;Furcht,LT;Vercellotti,GM
通讯作者：
Vercellotti,GM
影响因子：
56.9
作者：
THORNTON, SC;MUELLER, SN;LEVINE, EM
通讯作者：
LEVINE, EM
影响因子：
158.5
作者：
NEWBURGER, JW;TAKAHASHI, M;MELISH, ME
通讯作者：
MELISH, ME