Two Lysin-Motif Receptor Kinases, Gh-LYK1 and Gh-LYK2, Contribute to Resistance against Verticillium wilt in Upland Cotton.
Two Lysin-Motif Receptor Kinases, Gh-LYK1 and Gh-LYK2, Contribute to Resistance against Verticillium wilt in Upland Cotton.
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两种赖氨酸基序受体激酶 Gh-LYK1 和 Gh-LYK2 有助于陆地棉抵抗黄萎病
DOI：
10.3389/fpls.2017.02133
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发表时间：
2017
影响因子：
5.6
通讯作者：
Zhou X
中科院分区：
文献类型：
--
作者：
Gu Z;Liu T;Ding B;Li F;Wang Q;Qian S;Ye F;Chen T;Yang Y;Wang J;Wang G;Zhang B;Zhou X
赖氨酸基序（LysM）受体激酶（LYKs）在模式植物拟南芥和水稻中识别几丁质和激活对病原真菌的防御反应中起重要作用。然而，LYKs在非模式植物中的功能仍然是难以捉摸的。在本研究中，我们发现棉花中的两个LYK编码基因Gh-LYK 1和Gh-LYK 2在黄萎病菌感染后被诱导转录。棉花植物中病毒诱导的Gh-LYK 1和Gh-LYK 2基因沉默（VIGS）损害了对大丽轮枝菌的抗性。Gh-LYK 1和Gh-LYK 2作为模式识别受体（PRRs），均位于细胞膜上，其三个LysM结构域均是几丁质结合所必需的。然而，由于Gh-LYK 2是一种假激酶，而不是Gh-LYK 1，另一方面，Gh-LYK 2的胞外域（艾德）可以诱导植物体内活性氧（ROS）的爆发，Gh-LYK 2和Gh-LYK 1可能对棉花防御的贡献不同。综上所述，我们的研究结果表明，Gh-LYK 1和Gh-LYK 12都是棉花防御大丽轮枝菌所必需的，可能是通过不同的机制。
Lysin-motif (LysM) receptor kinases (LYKs) play essential roles in recognition of chitin and activation of defense responses against pathogenic fungi in the model plants Arabidopsis and rice. The function of LYKs in non-model plants, however, remains elusive. In the present work, we found that the transcription of two LYK-encoding genes from cotton, Gh-LYK1 and Gh-LYK2, was induced after Verticillium dahliae infection. Virus-induced gene silencing (VIGS) of Gh-LYK1 and Gh-LYK2 in cotton plants compromises resistance to V. dahliae. As putative pattern recognition receptors (PRRs), both Gh-LYK1 and Gh-LYK2 are membrane-localized, and all three LysM domains of Gh-LYK1 and Gh-LYK2 are required for their chitin-binding ability. However, since Gh-LYK2, but not Gh-LYK1, is a pseudo-kinase and, on the other hand, the ectodomain (ED) of Gh-LYK2 can induce reactive oxygen species (ROS) burst in planta, Gh-LYK2 and Gh-LYK1 may contribute differently to cotton defense. Taken together, our results establish that both Gh-LYK1 and Gh-LYK12 are required for defense against V. dahliae in cotton, possibly through different mechanisms.
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