The whole transcriptome regulation as a function of mitochondrial polymorphisms and aging in Caenorhabditis elegans
The whole transcriptome regulation as a function of mitochondrial polymorphisms and aging in Caenorhabditis elegans
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秀丽隐杆线虫线粒体多态性和衰老的全转录组调控
DOI：
10.18632/aging.102754
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发表时间：
2020-02
期刊：
影响因子：
--
通讯作者：
Zuobin Zhu
中科院分区：
文献类型：
--
作者：
Yuanjian Song;Yuechen Wang;Ying Li;Liang Wang;WenDa Zhang;Jing Cheng;Yao Zhu;Haoyu Zhang;Qiang Zhang;Haichen Niu;Yingwei zheng;Mengyu Liang;Mengqiong Deng;Hao Shi;Hao Wang;Fang Zhang;Zuobin Zhu
近年来，老化过程中的核-核相互作用得到了广泛的研究。然而，迄今为止，由影响衰老的自然线粒体变异控制的核基因组尚未得到全面了解。我们假设线粒体多态性可能在衰老过程中发挥关键作用，可能是通过调节全转录组表达。我们的研究结果表明，线粒体多态性不仅降低了线粒体质量，而且降低了miRNA、lncRNA、mRNA、circRNA和代谢产物谱。此外，大多数与线粒体相关的基因表现出年龄相关的表达特征（P = 3.58E-35）。我们还构建了一个差异表达的circRNA-lncRNA-miRNA-mRNA调控网络和一个受线粒体变异影响的ceRNA网络。此外，京都基因百科全书和基因组途径分析表明，受线粒体变异影响的基因富含代谢活性。最终构建了秀丽隐杆线虫线粒体变异对衰老的多层次调控网络。这些基因与代谢物之间的相互作用对于进一步的衰老研究具有重要价值。总之，我们的发现为理解线粒体如何影响衰老的分子机制提供了新的证据。
Recently, mitochondrial-nuclear interaction in aging has been widely studied. However, the nuclear genome controlled by natural mitochondrial variations that influence aging has not been comprehensively understood so far. We hypothesized that mitochondrial polymorphisms could play critical roles in the aging process, probably by regulation of the whole-transcriptome expression. Our results showed that mitochondria polymorphisms not only decreased the mitochondrial mass but also miRNA, lncRNA, mRNA, circRNA and metabolite profiles. Furthermore, most genes that are associated with mitochondria show age-related expression features (P = 3.58E-35). We also constructed a differentially expressed circRNA-lncRNA-miRNA-mRNA regulatory network and a ceRNA network affected by the mitochondrial variations. In addition, Kyoto Encyclopedia of Genes and Genomes pathway analyses showed that the genes affected by the mitochondrial variation were enriched in metabolic activity. We finally constructed a multi-level regulatory network with aging which affected by the mitochondrial variation in Caenorhabditis elegans. The interactions between these genes and metabolites have great values for further aging research. In sum, our findings provide new evidence for understanding the molecular mechanisms of how mitochondria influence aging.
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作者：
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通讯作者：
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影响因子：
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