Engineering a polymeric gene delivery vector based on poly(ethylenimine) and hyaluronic acid.
Engineering a polymeric gene delivery vector based on poly(ethylenimine) and hyaluronic acid.
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DOI：
10.1021/bm300145q
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发表时间：
2012-05-14
影响因子：
6.2
通讯作者：
Mikos, Antonios G.
中科院分区：
文献类型：
--
作者：
Needham, Clark J.;Williams, Austin K.;Chew, Sue Anne;Kasper, F. Kurtis;Mikos, Antonios G.
本文研究了伯胺、配体靶向和总电荷对支化聚亚胺-透明质酸偶联（bPEI-HA）两性离子基因传递载体有效性的影响。为了阐明这些参数的相对重要性，我们探索了各种bPEI-HA配方的zeta电位、细胞毒性和转染效率。结果表明，透明质酸（HA）寡糖的长度对细胞毒性和转染人间充质干细胞的效率影响最为显著。含10个糖段HA的bPEI试验组转染效率（14.6±2.0%）显著高于其他试验组，细胞毒性显著低于其他试验组，其中含10个糖段质量最大的试验组在最高聚合物浓度（100 μg/ml）下的细胞毒性与阳性对照相近。此外，相对于糖长度和HA质量的掺入，HA的摩尔掺入对zeta电位的影响最大，但对细胞毒性和转染效率的影响较小。这项工作证明了在创建两性离子聚合物基因传递载体时，这些可调设计标准的相对重要性，并提供了有关bPEI-HA基因传递载体设计的有用具体信息。
In this work, the effects of primary amines, ligand targeting, and overall charge on the effectiveness of branched poly(ethylenimine)-hyaluronic acid conjugate (bPEI-HA) zwitterionic gene delivery vectors are investigated. To elucidate the relative importance of each of these parameters, the zeta potential, cytotoxicity, and transfection efficiency were explored for a variety of formulations of bPEI-HA. It was found that the length of the hyaluronic acid (HA) oligosaccharide had the most significant effect on cytotoxicity and transfection efficiency with human mesenchymal stem cells. Test groups of bPEI incorporating HA with a length of 10 saccharides had significantly higher transfection efficiency (14.6±2.0%) and lower cytotoxicity than other formulations tested, with the cytotoxicity of the group containing the greatest mass of 10 saccharide showing similar results as the positive controls at the highest polymer concentration (100 μg/ml). Additionally, molar incorporation of HA, as opposed to the saccharide length and HA mass incorporation, had the greatest effect on zeta potential, but a minor effect on both cytotoxicity and transfection efficiency. This work demonstrates the relative importance of each of these tunable design criteria when creating a zwitterionic polymeric gene delivery vector and provides useful specific information regarding the design of bPEI-HA gene delivery vectors.
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影响因子：
4.9
作者：
Jiang, Ge;Park, Kitae;Hahn, Sei Kwang
通讯作者：
Hahn, Sei Kwang
DOI：
10.1097/01.blo.0000143804.26638.82
发表时间：
2004-10-01
影响因子：
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作者：
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作者：
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影响因子：
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作者：
Diebold, SS;Kursa, P;Zenke, M
通讯作者：
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