TMEM150C/Tentonin3 Is a Regulator of Mechano-gated Ion Channels.
TMEM150C/Tentonin3 Is a Regulator of Mechano-gated Ion Channels.
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DOI：
10.1016/j.celrep.2018.03.094
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发表时间：
2018-04-17
期刊：
影响因子：
8.8
通讯作者：
Bagriantsev SN
中科院分区：
文献类型：
--
作者：
Anderson EO;Schneider ER;Matson JD;Gracheva EO;Bagriantsev SN
神经元的机械敏感性依赖于机械化的离子通道，但调节其活动的途径仍然知之甚少。TMEM150C被认为介导本体感觉神经元的机械激活电流。在这里，我们用两种独立的机械刺激方法研究了TMEM150C与不同类别的机械门控离子通道(Piezo2、Piezo1和钾通道Trek-1)的功能相互作用。我们发现，TMEM150C显著延长了三种通道产生的机械电流的持续时间，降低了Piezo2的表观激活阈值，并在Piezo1中诱导了持续电流。我们还发现，TMEM150C与Piezo2在三叉神经细胞中共表达，将其作用扩展到本体感受器之外。最后，我们从触觉觅食家鸭的三叉神经细胞中克隆了TMEM150C，并表明它的功能与小鼠同源基因相似，证明了脊椎动物之间的进化保守。我们的研究表明，TMEM150C是不同类别的机械门控离子通道的通用调节器。机械门控离子通道是躯体感觉、本体感觉、听力、血管扩张和轴突生长所必需的。Anderson et al.结果表明，跨膜蛋白TMEM150C促进不同类别的机械门控离子通道的活性：PIEZO1/2和钾选择通道Trek-1。这项研究揭示了TMEM150C作为一种进化保守的机械敏感性调节因子的作用。
Neuronal mechano-sensitivity relies on mechanogated ion channels, but pathways regulating their activity remain poorly understood. TMEM150C was proposed to mediate mechano-activated current in proprioceptive neurons. Here, we studied functional interaction of TMEM150C with mechano-gated ion channels from different classes (Piezo2, Piezo1, and the potassium channel TREK-1) using two independent methods of mechanical stimulation. We found that TMEM150C significantly prolongs the duration of the mechano-current produced by all three channels, decreases apparent activation threshold in Piezo2, and induces persistent current in Piezo1. We also show that TMEM150C is co-expressed with Piezo2 in trigeminal neurons, expanding its role beyond proprioceptors. Finally, we cloned TMEM150C from the trigeminal neurons of the tactile-foraging domestic duck and showed that it functions similarly to the mouse ortholog, demonstrating evolutionary conservation among vertebrates. Our studies reveal TMEM150C as a general regulator of mechano-gated ion channels from different classes. Mechano-gated ion channels are essential for somatosensation, proprioception, hearing, vasodilation, and axonal growth. Anderson et al. show that the transmembrane protein TMEM150C facilitates activity of mechano-gated ion channels from different classes: Piezo1/2 and the potassium-selective channel TREK-1. This study reveals a role for TMEM150C as an evolutionarily conserved regulator of mechano-sensitivity.
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k（2p）2.1（Trek-1） - 激活器复合物揭示了一个隐秘的选择性滤波器结合位点。
影响因子：
64.8
作者：
Lolicato M;Arrigoni C;Mori T;Sekioka Y;Bryant C;Clark KA;Minor DL Jr
通讯作者：
Minor DL Jr
影响因子：
25
作者：
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通讯作者：
Franze K
影响因子：
7.7
作者：
Chung J;Nakatsu F;Baskin JM;De Camilli P
通讯作者：
De Camilli P
影响因子：
16.6
作者：
Cox CD;Bae C;Ziegler L;Hartley S;Nikolova-Krstevski V;Rohde PR;Ng CA;Sachs F;Gottlieb PA;Martinac B
通讯作者：
Martinac B
DOI：
10.1073/pnas.1407160111
发表时间：
2014-09-16
影响因子：
11.1
作者：
Comoglio, Yannick;Levitz, Joshua;Sandoz, Guillaume
通讯作者：
Sandoz, Guillaume