High-frequency transfer of a naturally occurring chromosomal tetracycline resistance element in the ruminal anaerobe Butyrivibrio fibrisolvens
High-frequency transfer of a naturally occurring chromosomal tetracycline resistance element in the ruminal anaerobe Butyrivibrio fibrisolvens
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瘤胃厌氧菌溶纤维丁酸弧菌中天然存在的染色体四环素抗性元件的高频转移
DOI：
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发表时间：
1997
影响因子：
4.4
通讯作者：
Harry J. Flint
中科院分区：
文献类型：
--
作者：
Karen P. Scott;Teresa M. Barbosa;Kenneth J. Forbes;Harry J. Flint
从牛瘤胃中分离到对四环素具有抗性的解纤丁酸弧菌。三分之二的Tcr B。测试的溶纤芽孢杆菌能够在厌氧过滤器配对中以每个供体细胞10（-7）至10（-1）的频率向溶纤芽孢杆菌B，2221 R的模式菌株的利福平抗性突变体提供四环素抗性。受体菌株2221 R表现出快速的自动聚集，这可能是观察到的高转移率的一个因素。B的TCR接合子。fibrisolvens 2221 R还能够进一步将四环素抗性转移到梭孢酸抗性突变体2221 F。脉冲场凝胶电泳的基因组DNA的比较表明，改变了transconjugants的频带配置文件，与收购一个大的移动的染色体元件一致。两个B的可转移元素。fibrisolvens供体1.23和1.230（分别为TnB 123和TnB 1230）在B中显示出相同的优选插入位点。fibrisolvens 2221 R染色体上，可能是相似或相同的元件。杂交实验表明TnB 1230与Tn 916或Tn 5253的int-xis区没有密切关系。虽然从B.在与携带泰特（M）或泰特（O）序列的探针杂交的纤维溶剂供体菌株中，发现转移接合物获得了与这些探针仅弱杂交的独特条带，表明第二个远缘相关的Tcr决定簇已经转移。
Butyrivibrio fibrisolvens strains resistant to tetracycline were isolated from the bovine rumen. Two of three Tcr B. fibrisolvens tested were able to donate tetracycline resistance at frequencies ranging from 10(-7) to 10(-1) per donor cell in anaerobic filter matings to a rifampin-resistant mutant of the type strain of B.fibrisolvens, 2221R. The recipient strain 2221R exhibited rapid autoaggregation, which might be a factor in the high transfer rates observed. Tcr transconjugants of B. fibrisolvens 2221R were also capable of further transferring tetracycline resistance to a fusidic acid-resistant mutant, 2221F. Comparison of genomic DNAs by pulsed-field gel electrophoresis demonstrated altered band profiles in transconjugants, consistent with the acquisition of a large mobile chromosomal element. The transferable elements from the two B. fibrisolvens donors 1.23 and 1.230 (TnB123 and TnB1230, respectively) showed the same preferred insertion site in the B. fibrisolvens 2221R chromosome and are likely to be similar, or identical, elements. Hybridization experiments showed no close relationship between TnB1230 and int-xis regions from Tn916 or Tn5253. Although DNA from the B. fibrisolvens donor strains hybridized with probes carrying tet(M) or tet(O) sequences, transconjugants were found to have acquired a distinct band that hybridized only weakly with these probes, suggesting that a second, distantly related Tcr determinant had been transferred.
影响因子：
15.9
作者：
CLEWELL, DB;FLANNAGAN, SE;JAWORSKI, DD
通讯作者：
JAWORSKI, DD