Novel biomarkers for post-contrast acute kidney injury identified from long non-coding RNA expression profiles.
Novel biomarkers for post-contrast acute kidney injury identified from long non-coding RNA expression profiles.
复制标题
从长非编码 RNA 表达谱中鉴定出对比后急性肾损伤的新型生物标志物
DOI：
10.7150/ijbs.45294
复制
发表时间：
2021
影响因子：
9.2
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Chen G;Liu B;Chen S;Li H;Liu J;Mai Z;Chen E;Zhou C;Sun G;Guo Z;Lei L;Huang S;Zhang L;Li M;Tan N;Li H;Liao Y;Liu J;Chen J;Liu Y
背景：对比剂后急性肾损伤（PC-AKI）是心导管插入术的严重并发症。新的证据表明，长链非编码RNA（lncRNA）可以作为各种疾病的生物标志物。然而，PC-AKI中的lncRNA表达谱和潜在生物标志物仍不清楚。本研究旨在研究用于PC-AKI早期检测的新型lncRNA生物标志物。方法：采用RNA测序技术检测PC-AKI大鼠肾组织中lncRNA的表达。通过人-大鼠同源性分析、大鼠肾脏和血液过滤鉴定潜在的lncRNA生物标志物，并在112个临床样本中验证。在HK-2细胞和大鼠模型中检测候选lncRNA的表达模式，以评估其早期检测的潜力。结果：共检测到357条lncRNA在PC-AKI中的差异表达。我们鉴定了lnc-HILPDA和lnc-PRND是保守的，并且在来自大鼠的肾脏和血液以及PC-AKI患者的血液中显著上调;这些lncRNA可以精确区分PC-AKI患者（曲线下面积（AUC）值分别为0.885和0.875）。这两种lncRNA的组合显示出预测PC-AKI的准确性提高，灵敏度为100%，特异性为83.93%。时程实验表明，在PC-AKI大鼠的血液中，lnc-HILPDA在12 h时和lnc-PRND在24 h时首先注意到显著差异。结论：我们的研究表明，lnc-HILPDA和lnc-PRND可以作为早期检测的新生物标志物，并深刻影响PC-AKI的临床分层和策略指导。
Background: Post-contrast acute kidney injury (PC-AKI) is a severe complication of cardiac catheterization. Emerging evidence indicated that long non-coding RNAs (lncRNAs) could serve as biomarkers for various diseases. However, the lncRNA expression profile and potential biomarkers in PC-AKI remain unclear. This study aimed to investigate novel lncRNA biomarkers for the early detection of PC-AKI. Methods: lncRNA profile in the kidney tissues of PC-AKI rats was evaluated through RNA sequencing. Potential lncRNA biomarkers were identified through human-rat homology analysis, kidney and blood filtering in rats and verified in 112 clinical samples. The expression patterns of the candidate lncRNAs were detected in HK-2 cells and rat models to evaluate their potential for early detection. Results: In total, 357 lncRNAs were found to be differentially expressed in PC-AKI. We identified lnc-HILPDA and lnc-PRND were conservative and remarkably upregulated in both kidneys and blood from rats and the blood of PC-AKI patients; these lncRNAs can precisely distinguish PC-AKI patients (area under the curve (AUC) values of 0.885 and 0.875, respectively). The combination of these two lncRNAs exhibited improved accuracy for predicting PC-AKI, with 100% sensitivity and 83.93% specificity. Time-course experiments showed that the significant difference was first noted in the blood of PC-AKI rats at 12 h for lnc-HILPDA and 24 h for lnc-PRND. Conclusion: Our study revealed that lnc-HILPDA and lnc-PRND may serve as the novel biomarkers for early detection and profoundly affect the clinical stratification and strategy guidance of PC-AKI.
登录
查看更多内容
DOI：
10.1038/nrm.2017.104
发表时间：
2018-03
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
Ransohoff JD;Wei Y;Khavari PA
通讯作者：
Khavari PA
WGCNA：用于加权相关网络分析的 R 包。
影响因子：
3
作者：
Langfelder P;Horvath S
通讯作者：
Horvath S
影响因子：
19.7
作者：
McDonald, Jennifer S.;McDonald, Robert J.;Williamson, Eric E.
通讯作者：
Williamson, Eric E.
影响因子：
4.5
作者：
Christiansen, C
通讯作者：
Christiansen, C
影响因子：
2.9
作者：
Edelstein, Charles L.
通讯作者：
Edelstein, Charles L.